ArticlePloS one2012
Genomic characterization of a novel virus of the family Tymoviridae isolated from mosquitoes.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 43 citations in OpenAlex.
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- Complete genome sequence of ramie marafivirus 1, a novel marafivirus infecting ramie (Boehmeria nivea).Archives of virology · 2026Article
- Diverse novel and avian-associated viruses in the ileal viromes of Northern Mockingbird (Mimus polyglottos).Archives of virology · 2026Article
- Complete genome sequence and genome organization of Ficus pumila-associated virus, a new putative member of the family Tymoviridae.Archives of virology · 2025Article
- VISTA: A Tool for Fast Taxonomic Assignment of Viral Genome Sequences.Genomics, proteomics & bioinformatics · 2025Article
- Sequence analysis and genome organization of a new marafivirus from Leptochloa chinensis.Archives of virology · 2025Article
- Bactericera cockerelli Picorna-like Virus and Three New Viruses Found Circulating in Populations of Potato/Tomato Psyllids (Viruses · 2024Article
- Identification of a novel mycovirus belonging to the "flexivirus"-related family with icosahedral virion.Virus evolution · 2024Article
- Complete genome sequence analysis of Valeriana jatamansi tymovirus 1: a novel member of the genus Tymovirus infecting Valeriana jatamansi Jones.Archives of virology · 2023Article
- Identification and Profiling of a NovelViruses · 2023Article
- Novel PutativeViruses · 2023Article
- Investigation of the Virome and Characterization of Issyk-Kul Virus from SwedishPathogens (Basel, Switzerland) · 2022Article
- Exploring the tymovirales landscape through metatranscriptomics data.Archives of virology · 2022Article
- Article
- Local-scale virome depiction in Medellín, Colombia, supports significant differences between Aedes aegypti and Aedes albopictus.PloS one · 2022Article
- Identification and characterisation of common glow-worm RNA viruses.Virus genes · 2020Article
- Viromics on Honey-Baited FTA Cards as a New Tool for the Detection of Circulating Viruses in Mosquitoes.Viruses · 2020Article
- The Use of Engineered Plant Viruses in aFrontiers in plant science · 2020Review
- Mosquito-Specific Viruses-Transmission and Interaction.Viruses · 2019Review
- Discovery of a novel Tymoviridae-like virus in mosquitoes from Mexico.Archives of virology · 2019Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe family Tymoviridae comprises three plant virus genera, including Tymovirus, Marafivirus, and Maculavirus, which are found in most parts of the world and cause severe agricultural losses. We describe a putatively novel member of the family Tymoviridae, which is isolated from mosquitoes (Culex spp.), referred to as CuTLV. METHODS AND
resultsThe CuTLV was isolated by cell culture, which replicates and causes cytopathic effects in Aedes albopictus C6/36 cells, but not in mammalian BHK-21 or Vero cells. The complete 6471 nucleotide sequence of CuTLV was determined. The genome of CuTLV is predicted to contain three open reading frames (ORFs). The largest ORF1 is 5307 nucleotides (nt) in length and encodes a putative polypeptide of 1769 amino acids (aa), which contains the conserved motifs for the methyltransferase (MTR), Tymovirus endopeptidase (PRO), helicase (HEL), and RNA-dependent RNA polymerase (RdRp) of the replication-associated proteins (RPs) of positive-stranded RNA viruses. In contrast, the ORF1 sequence does not contain the so-called "tymobox" or "marafibox", the conserved subgenomic RNA promoter present in all tymoviruses or marafiviruses, respectively. ORF2 and ORF3 putatively encode a 248-aa coat protein (CP) and a proline-rich 149-aa polypeptide. The whole genomic nucleotide identity of CuTLV with other members of family Tymoviridae ranged from 46.2% (ChiYMV) to 52.4% (GFkV). Phylogenetic analysis based on the putative RP and CP genes of CuTLV demonstrated that the virus is most closely related to viruses in the genus Maculavirus.
conclusionsThe CuTLV is a novel virus related to members of the family Tymoviridae, with molecular characters that are distinct from those of tymoviruses, marafiviruses, and other maculaviruses or macula-like viruses. This is the first report of the isolation of a Tymoviridae-like virus from mosquitoes. Further investigations are required to clarify the origin, replication strategy, and the public health or agricultural importance of the CuTLV.
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