ArticlePloS one2012
Identification of miRNA signatures during the differentiation of hESCs into retinal pigment epithelial cells.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Regulation of RPE65 expression in human retinal pigment epithelium cells.Scientific reports · 2025Article
- Small Extracellular Vesicle-Associated MiRNAs in Polarized Retinal Pigmented Epithelium.Investigative ophthalmology & visual science · 2024Article
- Retinal Pigment Epithelium Cell Development: Extrapolating Basic Biology to Stem Cell Research.Biomedicines · 2023Review
- Therapeutic effects of mesenchymal stem cells-derived extracellular vesicles' miRNAs on retinal regeneration: a review.Stem cell research & therapy · 2021Review
- Single nucleotide polymorphism rs13079080 is associated with differential regulation of the succinate receptor 1 (SUCNR1) gene by miRNA-4470.RNA biology · 2019Article
- Eye in a Disk: eyeIntegration Human Pan-Eye and Body Transcriptome Database Version 1.0.Investigative ophthalmology & visual science · 2019Article
- Overexpression of MiR-183/96/182 Triggers Retina-Like Fate in Human Bone Marrow-Derived Mesenchymal Stem Cells (hBMSCs) in Culture.Journal of ophthalmology · 2019Article
- Identifying core biological processes distinguishing human eye tissues with precise systems-level gene expression analyses and weighted correlation networks.Human molecular genetics · 2018Article
- Appropriately differentiated ARPE-19 cells regain phenotype and gene expression profiles similar to those of native RPE cells.Molecular vision · 2017Article
- MicroRNA biogenesis and their functions in regulating stem cell potency and differentiation.Biological procedures online · 2016Review
- Transdifferentiation of periodontal ligament-derived stem cells into retinal ganglion-like cells and its microRNA signature.Scientific reports · 2015Article
- miR-410 Inhibition Induces RPE Differentiation of Amniotic Epithelial Stem Cells via Overexpression of OTX2 and RPE65.Stem cell reviews and reports · 2015Article
- miRge - A Multiplexed Method of Processing Small RNA-Seq Data to Determine MicroRNA Entropy.PloS one · 2015Article
- Human pluripotent stem cell-derived retinal pigmented epithelium in retinal treatment: from bench to bedside.Molecular neurobiology · 2014Review
- MicroRNA expression profiles of human iPS cells, retinal pigment epithelium derived from iPS, and fetal retinal pigment epithelium.Journal of visualized experiments : JoVE · 2014Article
- Retinal pigment epithelium development, plasticity, and tissue homeostasis.Experimental eye research · 2014Review
- Transcription factor SOX9 plays a key role in the regulation of visual cycle gene expression in the retinal pigment epithelium.The Journal of biological chemistry · 2014Article
- MicroRNAs in the Neural Retina.International journal of genomics · 2014Review
- Profiling the microRNA Expression in Human iPS and iPS-derived Retinal Pigment Epithelium.Cancer informatics · 2014Article
- Article
Corrections and comments
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Authors and funding
15 authors at 5 institutions in 2 countries.
Funding
Abstract
Retinal pigment epithelium (RPE) cells can be obtained through in vitro differentiation of both embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). We have previously identified 87 signature genes relevant to RPE cell differentiation and function through transcriptome analysis of both human ESC- and iPSC-derived RPE as well as normal fetal RPE. Here, we profile miRNA expression through small RNA-seq in human ESCs and their RPE derivatives. Much like conclusions drawn from our previous transcriptome analysis, we find that the overall miRNA landscape in RPE is distinct from ESCs and other differentiated somatic tissues. We also profile miRNA expression during intermediate stages of RPE differentiation and identified unique subsets of miRNAs that are gradually up- or down-regulated, suggesting that dynamic regulation of these miRNAs is associated with the RPE differentiation process. Indeed, the down-regulation of a subset of miRNAs during RPE differentiation is associated with up-regulation of RPE-specific genes, such as RPE65, which is exclusively expressed in RPE. We conclude that miRNA signatures can be used to classify different degrees of in vitro differentiation of RPE from human pluripotent stem cells. We suggest that RPE-specific miRNAs likely contribute to the functional maturation of RPE in vitro, similar to the regulation of RPE-specific mRNA expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.