ArticleProceedings of the National Academy of Sciences of the United States of America2012
Multicopper oxidase-1 is a ferroxidase essential for iron homeostasis in Drosophila melanogaster.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 66 citations in OpenAlex.
- Effects of cuproptosis and its application in inflammatory bowel disease (Review).International journal of molecular medicine · 2026Review
- New molecular components of high and low affinity iron import systems in Drosophila.Nature communications · 2025Article
- Chromosome-level genomes of two Bracteacoccaceae highlight adaptations to biocrusts.Nature communications · 2025Article
- Gene expression variation underlying tissue-specific responses to copper stress in Drosophila melanogaster.G3 (Bethesda, Md.) · 2024Article
- Gene expression variation underlying tissue-specific responses to copper stress inbioRxiv : the preprint server for biology · 2023Article
- Iron Homeostasis in Insects.Annual review of entomology · 2023Review
- Key players in the regulation of iron homeostasis at the host-pathogen interface.Frontiers in immunology · 2023Review
- Synergistic and antagonistic interactions of oxybenzone and ocean acidification: new insight into vulnerable cellular processes in non-calcifying anthozoans.Frontiers in physiology · 2023Article
- Phenotypic analyses, protein localization, and bacteriostatic activity of Drosophila melanogaster transferrin-1.Insect biochemistry and molecular biology · 2022Article
- Mild chronic exposure to pesticides alters physiological markers of honey bee health without perturbing the core gut microbiota.Scientific reports · 2022Article
- Review
- Mosquito metallomics reveal copper and iron as critical factors for Plasmodium infection.PLoS neglected tropical diseases · 2021Article
- RNAi-Mediated Suppression ofFrontiers in physiology · 2020Article
- The Altered Expression ofFrontiers in plant science · 2019Article
- Anatomy and Physiology of the Digestive Tract ofGenetics · 2018Review
- Iron Sulfur and Molybdenum Cofactor Enzymes Regulate theFrontiers in physiology · 2018Article
- Drosophila melanogaster Models of Metal-Related Human Diseases and Metal Toxicity.International journal of molecular sciences · 2017Review
- Secretory laccase 1 in Bemisia tabaci MED is involved in whitefly-plant interaction.Scientific reports · 2017Article
- ZIP13: A Study ofFrontiers in genetics · 2017Review
- Ironing out the Details: Exploring the Role of Iron and Heme in Blood-Sucking Arthropods.Frontiers in physiology · 2017Review
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Multicopper ferroxidases catalyze the oxidation of ferrous iron to ferric iron. In yeast and algae, they participate in cellular uptake of iron; in mammals, they facilitate cellular efflux. The mechanisms of iron metabolism in insects are still poorly understood, and insect multicopper ferroxidases have not been identified. In this paper, we present evidence that Drosophila melanogaster multicopper oxidase-1 (MCO1) is a functional ferroxidase. We identified candidate iron-binding residues in the MCO1 sequence and found that purified recombinant MCO1 oxidizes ferrous iron. An association between MCO1 function and iron homeostasis was confirmed by two observations: RNAi-mediated knockdown of MCO1 resulted in decreased iron accumulation in midguts and whole insects, and weak knockdown increased the longevity of flies fed a toxic concentration of iron. Strong knockdown of MCO1 resulted in pupal lethality, indicating that MCO1 is an essential gene. Immunohistochemistry experiments demonstrated that MCO1 is located on the basal surfaces of the digestive system and Malpighian tubules. We propose that MCO1 oxidizes ferrous iron in the hemolymph and that the resulting ferric iron is bound by transferrin or melanotransferrin, leading to iron storage, iron withholding from pathogens, regulation of oxidative stress, and/or epithelial maturation. These proposed functions are distinct from those of other known ferroxidases. Given that MCO1 orthologues are present in all insect genomes analyzed to date, this discovery is an important step toward understanding iron metabolism in insects.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.