ArticleJournal of molecular and cellular cardiology2012
Features of endogenous cardiomyocyte chromatin revealed by super-resolution STED microscopy.
Article in Journal of molecular and cellular cardiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Single-cell transcriptomes in the heart: when every epigenome counts.Cardiovascular research · 2023Review
- Contribution of advanced fluorescence nano microscopy towards revealing mitotic chromosome structure.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2021Review
- Super-Resolution Microscopy of Chromatin.Genes · 2019Review
- Direct visualization of cardiac transcription factories reveals regulatory principles of nuclear architecture during pathological remodeling.Journal of molecular and cellular cardiology · 2019Article
- Exploiting the tunability of stimulated emission depletion microscopy for super-resolution imaging of nuclear structures.Nature communications · 2018Article
- The march of pluripotent stem cells in cardiovascular regenerative medicine.Stem cell research & therapy · 2018Review
- Super-Resolution Imaging of Higher-Order Chromatin Structures at Different Epigenomic States in Single Mammalian Cells.Cell reports · 2018Article
- Epigenomes in Cardiovascular Disease.Circulation research · 2018Review
- Single-molecule fluorescence microscopy review: shedding new light on old problems.Bioscience reports · 2017Review
- Tricellular junctions regulate intestinal stem cell behaviour to maintain homeostasis.Nature cell biology · 2017Article
- The chromatin-binding protein Smyd1 restricts adult mammalian heart growth.American journal of physiology. Heart and circulatory physiology · 2016Article
- Reciprocal Regulation of the Cardiac Epigenome by Chromatin Structural Proteins Hmgb and Ctcf: IMPLICATIONS FOR TRANSCRIPTIONAL REGULATION.The Journal of biological chemistry · 2016Article
- Super-resolution microscopy reveals decondensed chromatin structure at transcription sites.Scientific reports · 2014Article
- Regulation of chromatin structure in the cardiovascular system.Circulation journal : official journal of the Japanese Circulation Society · 2013Review
- Structural considerations for chromatin state models with transcription as a functional readout.FEBS letters · 2012Article
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Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Despite the extensive knowledge of the functional unit of chromatin-the nucleosome-for which structural information exists at the atomic level, little is known about the endogenous structure of eukaryotic genomes. Chromosomal capture techniques and genome-wide chromatin immunoprecipitation and next generation sequencing have provided complementary insight into global features of chromatin structure, but these methods do not directly measure structural features of the genome in situ. This lack of insight is particularly troublesome in terminally differentiated cells which must reorganize their genomes for large scale gene expression changes in the absence of cell division. For example, cardiomyocytes, which are fully committed and reside in interphase, are capable of massive gene expression changes in response to physiological stimuli, but the global changes in chromatin structure that enable such transcriptional changes are unknown. The present study addressed this problem utilizing super-resolution stimulated emission depletion (STED) microscopy to directly measure chromatin features in mammalian cells. We demonstrate that immunolabeling of histone H3 coupled with STED imaging reveals chromatin domains on a scale of 40-70 nm, several folds better than the resolution of conventional confocal microscopy. An analytical workflow is established to detect changes in chromatin structure following acute stimuli and used to investigate rearrangements in cardiomyocyte genomes following agonists that induce cellular hypertrophy. This approach is readily adaptable to investigation of other nuclear features using a similar antibody-based labeling technique and enables direct measurements of chromatin domain changes in response to physiological stimuli.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.