ArticleBMC genomics2012
The RosR transcription factor is required for gene expression dynamics in response to extreme oxidative stress in a hypersaline-adapted archaeon.
Article in BMC genomics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 44 citations in OpenAlex.
- Investigation of the global translational response to oxidative stress in the model archaeonmSphere · 2025Article
- Rapid rewiring of an archaeal transcription factor function via flexible cis-trans interactions.Molecular biology of the cell · 2025Article
- Navigating the archaeal frontier: insights and projections from bioinformatic pipelines.Frontiers in microbiology · 2024Article
- TrmB Family Transcription Factor as a Thiol-Based Regulator of Oxidative Stress Response.mBio · 2022Article
- Post-transcriptional regulation of redox homeostasis by the small RNA SHOxi in haloarchaea.RNA biology · 2021Article
- The Survival ofMicroorganisms · 2021Article
- Review
- Carotenoids as a Protection Mechanism against Oxidative Stress inAntioxidants (Basel, Switzerland) · 2020Article
- A Bayesian non-parametric mixed-effects model of microbial growth curves.PLoS computational biology · 2020Article
- The Role of Stress Proteins in Haloarchaea and Their Adaptive Response to Environmental Shifts.Biomolecules · 2020Review
- Article
- Global Transcriptional Programs in Archaea Share Features with the Eukaryotic Environmental Stress Response.Journal of molecular biology · 2019Review
- Specificity of protein-DNA interactions in hypersaline environment: structural studies on complexes of Halobacterium salinarum oxidative stress-dependent protein hsRosR.Nucleic acids research · 2019Article
- Article
- Conserved principles of transcriptional networks controlling metabolic flexibility in archaea.Emerging topics in life sciences · 2018Article
- Methionine Sulfoxide Reductases of Archaea.Antioxidants (Basel, Switzerland) · 2018Review
- Copy number variation is associated with gene expression change in archaea.Microbial genomics · 2018Article
- Transcriptional Landscape and Regulatory Roles of Small Noncoding RNAs in the Oxidative Stress Response of the Haloarchaeon Haloferax volcanii.Journal of bacteriology · 2018Article
- Clustering gene expression time series data using an infinite Gaussian process mixture model.PLoS computational biology · 2018Article
- A transcription network of interlocking positive feedback loops maintains intracellular iron balance in archaea.Nucleic acids research · 2017Article
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Authors and funding
4 authors at 2 institutions in 3 countries.
Funding
Abstract
backgroundPrevious work has shown that the hypersaline-adapted archaeon, Halobacterium salinarum NRC-1, is highly resistant to oxidative stress caused by exposure to hydrogen peroxide, UV, and gamma radiation. Dynamic alteration of the gene regulatory network (GRN) has been implicated in such resistance. However, the molecular functions of transcription regulatory proteins involved in this response remain unknown.
resultsHere we have reanalyzed several existing GRN and systems biology datasets for H. salinarum to identify and characterize a novel winged helix-turn-helix transcription factor, VNG0258H, as a regulator required for reactive oxygen species resistance in this organism. This protein appears to be unique to the haloarchaea at the primary sequence level. High throughput quantitative growth assays in a deletion mutant strain implicate VNG0258H in extreme oxidative stress resistance. According to time course gene expression analyses, this transcription factor is required for the appropriate dynamic response of nearly 300 genes to reactive oxygen species damage from paraquat and hydrogen peroxide. These genes are predicted to function in repair of oxidative damage to proteins and DNA. In vivo DNA binding assays demonstrate that VNG0258H binds DNA to mediate gene regulation.
conclusionsTogether these results suggest that VNG0258H is a novel archaeal transcription factor that regulates gene expression to enable adaptation to the extremely oxidative, hypersaline niche of H. salinarum. We have therefore renamed VNG0258H as RosR, for reactive oxygen species regulator.
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