ArticleBMC genomics2012
Genome-wide expression analysis upon constitutive activation of the HacA bZIP transcription factor in Aspergillus niger reveals a coordinated cellular response to counteract ER stress.
Article in BMC genomics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 83 citations in OpenAlex.
- Integrative perspectives on glycosylation networks in fungi and oomycetes.Journal of microbiology (Seoul, Korea) · 2025Review
- Dual Role of MtHAC-1 in Regulating Cellulase and Xylanase Production in Myceliophthora thermophila.Microbial biotechnology · 2025Article
- Protein secretion and associated stress in industrially employed filamentous fungi.Applied microbiology and biotechnology · 2024Review
- TheJournal of fungi (Basel, Switzerland) · 2024Article
- Enhancement of protein production in Aspergillus niger by engineering the antioxidant defense metabolism.Biotechnology for biofuels and bioproducts · 2024Article
- Effect of the res2 transcription factor gene deletion on protein secretion and stress response in the hyperproducer strain Trichoderma reesei Rut-C30.BMC microbiology · 2023Article
- Physiological ER stress caused by amylase production induces regulated Ire1-dependent mRNA decay in Aspergillus oryzae.Communications biology · 2023Article
- Systematic Characterization of bZIP Transcription Factors Required for Development and Aflatoxin Generation by High-Throughput Gene Knockout inJournal of fungi (Basel, Switzerland) · 2022Article
- From induction to secretion: a complicated route for cellulase production in Trichoderma reesei.Bioresources and bioprocessing · 2021Review
- Profiling of chromatin accessibility identifies transcription factor binding sites across the genome of Aspergillus species.BMC biology · 2021Article
- Comparative transcriptome analysis of Trichoderma reesei reveals different gene regulatory networks induced by synthetic mixtures of glucose and β-disaccharide.Bioresources and bioprocessing · 2021Article
- Unfolded Protein Response and Scaffold Independent Pheromone MAP Kinase Signaling ControlJournal of fungi (Basel, Switzerland) · 2021Article
- Article
- Expression of SidD gene and physiological characterization of the rhizosphere plant growth-promoting yeasts.Heliyon · 2020Article
- Improvement of homologous GH10 xylanase production by deletion of genes with predicted function in the Aspergillus nidulans secretion pathway.Microbial biotechnology · 2020Article
- Genetic Engineering of Filamentous Fungi for Efficient Protein Expression and Secretion.Frontiers in bioengineering and biotechnology · 2020Review
- Signal peptide peptidase activity connects the unfolded protein response to plant defense suppression by Ustilago maydis.PLoS pathogens · 2019Article
- Deletion of the small GTPaseFungal biology and biotechnology · 2019Article
- Protein hyperproduction in fungi by design.Applied microbiology and biotechnology · 2018Review
- Construction of an improved Aspergillus niger platform for enhanced glucoamylase secretion.Microbial cell factories · 2018Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 4 countries.
Funding
Abstract
backgroundHacA/Xbp1 is a conserved bZIP transcription factor in eukaryotic cells which regulates gene expression in response to various forms of secretion stress and as part of secretory cell differentiation. In the present study, we replaced the endogenous hacA gene of an Aspergillus niger strain with a gene encoding a constitutively active form of the HacA transcription factor (HacACA). The impact of constitutive HacA activity during exponential growth was explored in bioreactor controlled cultures using transcriptomic analysis to identify affected genes and processes.
resultsTranscription profiles for the wild-type strain (HacAWT) and the HacACA strain were obtained using Affymetrix GeneChip analysis of three replicate batch cultures of each strain. In addition to the well known HacA targets such as the ER resident foldases and chaperones, GO enrichment analysis revealed up-regulation of genes involved in protein glycosylation, phospholipid biosynthesis, intracellular protein transport, exocytosis and protein complex assembly in the HacACA mutant. Biological processes over-represented in the down-regulated genes include those belonging to central metabolic pathways, translation and transcription. A remarkable transcriptional response in the HacACA strain was the down-regulation of the AmyR transcription factor and its target genes.
conclusionsThe results indicate that the constitutive activation of the HacA leads to a coordinated regulation of the folding and secretion capacity of the cell, but with consequences on growth and fungal physiology to reduce secretion stress.
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