ArticlePlant physiology2012
Antisense reduction of NADP-malic enzyme in Flaveria bidentis reduces flow of CO2 through the C4 cycle.
Article in Plant physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- Antisense reduction in NADP-ME in the CThe New phytologist · 2026Article
- Agrobacterium-mediated transient transformation of Flaveria bidentis leaves: a novel method to examine the evolution of CPlant methods · 2024Article
- Photosynthesis and food security: the evolving story of CPhotosynthesis research · 2023Review
- Installation of CPlant biotechnology journal · 2021Article
- Bundle sheath suberisation is required for CCommunications biology · 2021Article
- Diffusion of COPlant physiology · 2018Article
- Shade compromises the photosynthetic efficiency of NADP-ME less than that of PEP-CK and NAD-ME C4 grasses.Journal of experimental botany · 2018Article
- The Rubisco Chaperone BSD2 May Regulate Chloroplast Coverage in Maize Bundle Sheath Cells.Plant physiology · 2017Article
- Short-term thermal photosynthetic responses of C4 grasses are independent of the biochemical subtype.Journal of experimental botany · 2017Article
- Bundle-sheath leakiness and intrinsic water use efficiency of a perennial C4 grass are increased at high vapour pressure deficit during growth.Journal of experimental botany · 2017Article
- Effects of reduced carbonic anhydrase activity on CO2 assimilation rates in Setaria viridis: a transgenic analysis.Journal of experimental botany · 2017Article
- Carbon isotope discrimination as a diagnostic tool for C4 photosynthesis in C3-C4 intermediate species.Journal of experimental botany · 2016Article
- Improved analysis of C4 and C3 photosynthesis via refined in vitro assays of their carbon fixation biochemistry.Journal of experimental botany · 2016Article
- Photosynthesis of C3, C3-C4, and C4 grasses at glacial CO2.Journal of experimental botany · 2014Article
- Photosynthetic flexibility in maize exposed to salinity and shade.Journal of experimental botany · 2014Article
- Transplastomic integration of a cyanobacterial bicarbonate transporter into tobacco chloroplasts.Journal of experimental botany · 2014Article
- Elements required for an efficient NADP-malic enzyme type C4 photosynthesis.Plant physiology · 2014Article
- The coordination of C4 photosynthesis and the CO2-concentrating mechanism in maize and Miscanthus x giganteus in response to transient changes in light quality.Plant physiology · 2014Article
- Temperature response of photosynthesis in C3, C4, and CAM plants: temperature acclimation and temperature adaptation.Photosynthesis research · 2014Review
- Getting the most out of natural variation in C4 photosynthesis.Photosynthesis research · 2014Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An antisense construct targeting the C(4) isoform of NADP-malic enzyme (ME), the primary enzyme decarboxylating malate in bundle sheath cells to supply CO(2) to Rubisco, was used to transform the dicot Flaveria bidentis. Transgenic plants (α-NADP-ME) exhibited a 34% to 75% reduction in NADP-ME activity relative to the wild type with no visible growth phenotype. We characterized the effect of reducing NADP-ME on photosynthesis by measuring in vitro photosynthetic enzyme activity, gas exchange, and real-time carbon isotope discrimination (Δ). In α-NADP-ME plants with less than 40% of wild-type NADP-ME activity, CO(2) assimilation rates at high intercellular CO(2) were significantly reduced, whereas the in vitro activities of both phosphoenolpyruvate carboxylase and Rubisco were increased. Δ measured concurrently with gas exchange in these plants showed a lower Δ and thus a lower calculated leakiness of CO(2) (the ratio of CO(2) leak rate from the bundle sheath to the rate of CO(2) supply). Comparative measurements on antisense Rubisco small subunit F. bidentis plants showed the opposite effect of increased Δ and leakiness. We use these measurements to estimate the C(4) cycle rate, bundle sheath leak rate, and bundle sheath CO(2) concentration. The comparison of α-NADP-ME and antisense Rubisco small subunit demonstrates that the coordination of the C(3) and C(4) cycles that exist during environmental perturbations by light and CO(2) can be disrupted through transgenic manipulations. Furthermore, our results suggest that the efficiency of the C(4) pathway could potentially be improved through a reduction in C(4) cycle activity or increased C(3) cycle activity.
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