ArticlePloS one2012
Rapid detection and identification of human hookworm infections through high resolution melting (HRM) analysis.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Identification of Acantamoeba isolated from water resources located in the northwest of Iran by using the high-resolution melting analysis assay.International microbiology : the official journal of the Spanish Society for Microbiology · 2026Article
- Evolution ofJournal of parasitology research · 2026Review
- Comparison of Next-Generation Sequencing, Real-Time PCR and HRM-PCR forMicroorganisms · 2025Article
- Haplotype and Presence-Absence Variation Analysis Using High-Resolution Melting (HRM) Analysis for Genotyping Genes of Interest.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Current advances in serological and molecular diagnosis of Schistosoma mekongi infection.Tropical medicine and health · 2024Review
- Cultivation and sequencing-free protocol foriScience · 2024Article
- Application of Nested-qPCR-High Resolution Melting (HRM) Technology on Strongyloides stercoralis Isolates from Iran.Acta parasitologica · 2024Article
- Establishment of a Simple and Rapid Nucleic Acid Detection Method for Hookworm Identification.Pathogens (Basel, Switzerland) · 2023Article
- Rapid Detection and Identification ofIranian journal of public health · 2023Article
- Advantages and Limitations of Microscopy and Molecular Detections for Diagnosis of Soil-transmitted Helminths: An Overview.Helminthologia · 2022Article
- Article
- Potentially zoonotic gastrointestinal nematodes co-infecting free ranging non-human primates in Kenyan urban centres.Veterinary medicine and science · 2021Article
- Development of a New LAMP Assay for the Detection ofFrontiers in veterinary science · 2021Article
- Application of High-Resolution Melting (HRM) Technique towards the Detection of Asymptomatic Malaria in a Malaria Endemic Area of Southeastern Iran under Elimination Program.Journal of arthropod-borne diseases · 2020Article
- Recent advances in nucleic acid-based methods for detection of helminth infections and the perspective of biosensors for future development.Parasitology · 2020Review
- Egg genotyping reveals the possibility of patent Ancylostoma caninum infection in human intestine.Scientific reports · 2020Article
- High-Resolution Melting Analysis as an Appropriate Method to Differentiate betweenIranian journal of public health · 2019Article
- Establishment of a Tm-shift Method for Detection of Cat-Derived Hookworms.The Korean journal of parasitology · 2019Article
- Article
- Soil-Transmitted Helminths in Tropical Australia and Asia.Tropical medicine and infectious disease · 2017Review
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundHookworm infections are still endemic in low and middle income tropical countries with greater impact on the socioeconomic and public health of the bottom billion of the world's poorest people. In this study, a real-time polymerase chain reaction (PCR) coupled with high resolution melting-curve (HRM) analysis was evaluated for an accurate, rapid and sensitive tool for species identification focusing on the five human hookworm species.
methodsReal-time PCR coupled with HRM analysis targeting the second internal transcribed spacer (ITS-2) of nuclear ribosomal DNA as the genetic marker was used to identify and distinguish hookworm species in human samples. Unique and distinct characteristics of HRM patterns were produced for each of the five hookworm species. The melting curves were characterized by peaks of 79.24±0.05°C and 83.00±0.04°C for Necator americanus, 79.12±0.10°C for Ancylostoma duodenale, 79.40±0.10°C for Ancylostoma ceylanicum, 79.63±0.05°C for Ancylostoma caninum and 79.70±0.14°C for Ancylostoma braziliense. An evaluation of the method's sensitivity and specificity revealed that this assay was able to detect as low as 0.01 ng/µl hookworm DNA and amplification was only recorded for hookworm positive samples.
conclusionThe HRM assay developed in this study is a rapid and straightforward method for the diagnosis, identification and discrimination of five human hookworms. This assay is simple compared to other probe-based genotyping methods as it does not require multiplexing, DNA sequencing or post-PCR processing. Therefore, this method offers a new alternative for rapid detection of human hookworm species.
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