ArticlePloS one2012
The Ca2+/calcineurin-dependent signaling pathway in the gray mold Botrytis cinerea: the role of calcipressin in modulating calcineurin activity.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 43 citations in OpenAlex.
- A multivalent docking platform and Rcn1-mediated inhibition control the extent of calcineurin recruitment to the cell division site for the dephosphorylation of multiple cytokinetic proteins.bioRxiv : the preprint server for biology · 2026Article
- Combined biocontrol to tomato gray mold and inhibition via multi-mechanistic antifungal activity to Botrytis cinerea by Trichoderma harzianum T4.Archives of microbiology · 2026Article
- Polygenic strategies for host-specific and general virulence of Botrytis cinerea across diverse eudicot hosts.Genetics · 2025Article
- Transcriptome Analysis Provides Insights into the Mechanism of the Transcription FactorInternational journal of molecular sciences · 2024Article
- Nitric Oxide Metabolism Affects Germination inJournal of fungi (Basel, Switzerland) · 2022Article
- A Calcineurin Regulator MoRCN1 Is Important for Asexual Development, Stress Response, and Plant Infection ofFrontiers in plant science · 2022Article
- Cyclophilin BcCyp2 Regulates Infection-Related Development to Facilitate Virulence of the Gray Mold FungusInternational journal of molecular sciences · 2021Article
- The Destructive Fungal PathogenPathogens (Basel, Switzerland) · 2020Review
- Calcineurin Regulates Conidiation, Chlamydospore Formation and Virulence inFrontiers in microbiology · 2020Article
- Proteomic study of the membrane components of signalling cascades of Botrytis cinerea controlled by phosphorylation.Scientific reports · 2019Article
- Disruption of calcineurin catalytic subunit (cnaA) in Epichloë festucae induces symbiotic defects and intrahyphal hyphae formation.Molecular plant pathology · 2018Article
- Article
- Proteomic Analysis of Kiwifruit in Response to the Postharvest Pathogen,Frontiers in plant science · 2018Article
- The Protein Disulfide Isomerase ofFrontiers in microbiology · 2017Article
- The Trichoderma atroviride putative transcription factor Blu7 controls light responsiveness and tolerance.BMC genomics · 2016Article
- BcMctA, a putative monocarboxylate transporter, is required for pathogenicity in Botrytis cinerea.Current genetics · 2015Article
- The signalling mucin Msb2 regulates surface sensing and host penetration via BMP1 MAP kinase signalling in Botrytis cinerea.Molecular plant pathology · 2015Article
- Functional analysis of BcBem1 and its interaction partners in Botrytis cinerea: impact on differentiation and virulence.PloS one · 2014Article
- Involvement of Botrytis cinerea small GTPases BcRAS1 and BcRAC in differentiation, virulence, and the cell cycle.Eukaryotic cell · 2013Article
- Molecular identification of melanised non-sporulating moulds: a useful tool for studying the epidemiology of phaeohyphomycosis.Mycopathologia · 2013Article
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Authors and funding
3 authors at 1 institution in 1 country.
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Abstract
In the gray mold fungus Botrytis cinerea the Gα subunit Bcg1 of a heterotrimeric G protein is an upstream activator of the Ca(2+)/calmodulin-dependent phosphatase calcineurin. In this study we focused on the functional characterization of the catalytic subunit of calcineurin (BcCnA) and its putative regulator calcipressin (BcRcn1). We deleted the genes encoding both proteins to examine their role concerning growth, differentiation and virulence. The ΔbccnA mutant shows a severe growth defect, does not produce conidia and is avirulent, while the loss of BcRcn1 caused retardation of hyphal growth and delayed infection of host plants, but had no impact on conidiation and sclerotia formation. Expression of several calcineurin-dependent genes and bccnA itself is positively affected by BcRcn1. Complementation of the Δbcrcn1 mutant with a GFP-BcRcn1 fusion construct revealed that BcRcn1 is localized in the cytoplasm and accumulates around the nuclei. Furthermore, we showed that BcCnA physically interacts with BcRcn1 and the regulatory subunit of calcineurin, BcCnB. We investigated the impact of several protein domains characteristic for modulation and activation of BcCnA via BcRcn1, such as the phosphorylation sites and the calcineurin-docking site, by physical interaction studies between BcCnA and wild-type and mutated copies of BcRcn1. Based on the observed phenotypes we conclude that BcRcn1 acts as a positive modulator of BcCnA and the Ca(2+)/calcineurin-mediated signal transduction in B. cinerea, and that both proteins regulate fungal development and virulence.
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