ArticleNucleic acids research2012
The role of Ca²⁺ in the activity of Mycobacterium tuberculosis DNA gyrase.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 23 citations in OpenAlex.
- AnaToc75 (Alr2269) mediates calcium uptake across the outer membrane inJournal of bacteriology · 2025Article
- Toxin release by conditional remodelling of ParDE1 from Mycobacterium tuberculosis leads to gyrase inhibition.Nucleic acids research · 2024Article
- Spiropyrimidinetriones: a Class of DNA Gyrase Inhibitors with Activity against Mycobacterium tuberculosis and without Cross-Resistance to Fluoroquinolones.Antimicrobial agents and chemotherapy · 2022Article
- The pentapeptide-repeat protein, MfpA, interacts with mycobacterial DNA gyrase as a DNA T-segment mimic.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Structural and mechanistic analysis of ATPase inhibitors targeting mycobacterial DNA gyrase.The Journal of antimicrobial chemotherapy · 2020Article
- Repurposing Dihydropyridines for Treatment ofPharmaceutics · 2019Article
- Bioinformatic Exploration of Metal-Binding Proteome of Zoonotic PathogenFrontiers in genetics · 2019Article
- Article
- Plasmodium falciparum apicoplast and its transcriptional regulation through calcium signaling.Journal of microbiology (Seoul, Korea) · 2017Review
- Predictive modeling targets thymidylate synthase ThyX in Mycobacterium tuberculosis.Scientific reports · 2016Article
- DNA Gyrase of Deinococcus radiodurans is characterized as Type II bacterial topoisomerase and its activity is differentially regulated by PprA in vitro.Extremophiles : life under extreme conditions · 2016Article
- Fluoroquinolones stimulate the DNA cleavage activity of topoisomerase IV by promoting the binding of Mg(2+) to the second metal binding site.Biochimica et biophysica acta · 2016Article
- Functional Analyses of the Toxoplasma gondii DNA Gyrase Holoenzyme: A Janus Topoisomerase with Supercoiling and Decatenation Abilities.Scientific reports · 2015Article
- Article
- Unique features of apicoplast DNA gyrases from Toxoplasma gondii and Plasmodium falciparum.BMC bioinformatics · 2014Article
- Molecular complementarity between simple, universal molecules and ions limited phenotype space in the precursors of cells.Biology direct · 2014Article
- Identification of the likely translational start of Mycobacterium tuberculosis GyrB.BMC research notes · 2013Article
- The naphthoquinone diospyrin is an inhibitor of DNA gyrase with a novel mechanism of action.The Journal of biological chemistry · 2013Article
- Antimicrobial activity of novel c2-substituted 1,4-dihydropyridine analogues.Scientia pharmaceuticaArticle
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
DNA gyrase is the only type II topoisomerase in Mycobacterium tuberculosis and needs to catalyse DNA supercoiling, relaxation and decatenation reactions in order to fulfil the functions normally carried out by gyrase and DNA topoisomerase IV in other bacteria. We have obtained evidence for the existence of a Ca(2+)-binding site in the GyrA subunit of M. tuberculosis gyrase. Ca(2+) cannot support topoisomerase reactions in the absence of Mg(2+), but partial removal of Ca(2+) from GyrA by dialysis against EGTA leads to a modest loss in relaxation activity that can be restored by adding back Ca(2+). More extensive removal of Ca(2+) by denaturation of GyrA and dialysis against EGTA results in an enzyme with greatly reduced enzyme activities. Mutation of the proposed Ca(2+)-binding residues also leads to loss of activity. We propose that Ca(2+) has a regulatory role in M. tuberculosis gyrase and suggest a model for the modulation of gyrase activity by Ca(2+) binding.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.