ArticleApplied and environmental microbiology2012
Construction and application of a luxABCDE reporter system for real-time monitoring of Enterococcus faecalis gene expression and growth.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 25 citations in OpenAlex.
- Development of bioluminescent Group B streptococcal strains for longitudinal infection studies.Scientific reports · 2024Article
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- Monitoring Gene Expression during aMicroorganisms · 2020Article
- Immunotherapy with engineered bacteria by targeting the STING pathway for anti-tumor immunity.Nature communications · 2020Article
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- In vivo activity and low toxicity of the second-generation antimicrobial peptide DGL13K.PloS one · 2019Article
- Recent Advances in the Use ofJournal of fungi (Basel, Switzerland) · 2018Review
- In-vivo monitoring of infectious diseases in living animals using bioluminescence imaging.Virulence · 2018Review
- Rapid production and characterization of antimicrobial colicins usingSynthetic biology (Oxford, England) · 2018Article
- Article
- Galleria mellonella infection models for the study of bacterial diseases and for antimicrobial drug testing.Virulence · 2016Review
- The Basics of Bacteriuria: Strategies of Microbes for Persistence in Urine.Frontiers in cellular and infection microbiology · 2016Review
- Bioluminescence based biosensors for quantitative detection of enterococcal peptide-pheromone activity reveal inter-strain telesensing in vivo during polymicrobial systemic infection.Scientific reports · 2015Article
- Article
- Transcriptomic and functional analysis of NaCl-induced stress in Enterococcus faecalis.PloS one · 2014Article
- In vivo assessment of growth and virulence gene expression during commensal and pathogenic lifestyles of luxABCDE-tagged Enterococcus faecalis strains in murine gastrointestinal and intravenous infection models.Applied and environmental microbiology · 2013Article
- Non-invasive monitoring of Streptococcus pyogenes vaccine efficacy using biophotonic imaging.PloS one · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The present work describes the construction of a novel molecular tool for luciferase-based bioluminescence (BL) tagging of Enterococcus faecalis. To this end, a vector (pSL101) and its derivatives conferring a genetically encoded bioluminescent phenotype on all tested strains of E. faecalis were constructed. pSL101 harbors the luxABCDE operon from pPL2lux and the pREG696 broad-host-range replicon and axe-txe toxin-antitoxin cassette, providing segregational stability for long-term plasmid persistence in the absence of antibiotic selection. The bioluminescent signals obtained from three highly expressed promoters correlated linearly (R(2) > 0.98) with the viable-cell count. We employed lux-tagged E. faecalis strains to monitor growth in real time in milk and urine in vitro. Furthermore, bioluminescence imaging (BLI) was used to visualize the magnitude of the bacterial burden during infection in the Galleria mellonella model system. To our knowledge, pSL101 is the first substrate addition-independent reporter system developed for BLI of E. faecalis and an efficient tool for spatiotemporal tracking of bacterial growth and quantitative determination of promoter activity in real time, noninvasively, in infection model systems.
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