ArticleJournal of materials science. Materials in medicine2012
Overcoming hypoxia in 3D culture systems for tissue engineering of bone in vitro using an automated, oxygen-triggered feedback loop.
Article in Journal of materials science. Materials in medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Imaging Oxygen Concentrations in Bone Scaffolds during Cellular Activity and Fluid Perfusion.ACS biomaterials science & engineering · 2025Article
- The one-step fabrication of porous hASC-laden GelMa constructs using a handheld printing system.NPJ Regenerative medicine · 2023Article
- Chondrocytes In Vitro Systems Allowing Study of OA.International journal of molecular sciences · 2022Review
- Perfused Platforms to Mimic Bone Microenvironment at the Macro/Milli/Microscale: Pros and Cons.Frontiers in cell and developmental biology · 2021Review
- A Perfusion Bioreactor System for Cell Seeding and Oxygen-Controlled Cultivation of Three-Dimensional Cell Cultures.Tissue engineering. Part C, Methods · 2018Article
- Oxygen mapping: Probing a novel seeding strategy for bone tissue engineering.Biotechnology and bioengineering · 2017Article
- Indirect three-dimensional printing: A method for fabricating polyurethane-urea based cardiac scaffolds.Journal of biomedical materials research. Part A · 2016Article
- Bridging the gap between traditional cell cultures and bioreactors applied in regenerative medicine: practical experiences with the MINUSHEET perfusion culture system.Cytotechnology · 2016Article
- Influence of Different Three-Dimensional Open Porous Titanium Scaffold Designs on Human Osteoblasts Behavior in Static and Dynamic Cell Investigations.Materials (Basel, Switzerland) · 2015Article
- In Vitro Evaluation of Scaffolds for the Delivery of Mesenchymal Stem Cells to Wounds.BioMed research international · 2015Article
- Noninvasive Oxygen Monitoring in Three-Dimensional Tissue Cultures Under Static and Dynamic Culture Conditions.BioResearch open access · 2015Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tissue engineering is an attractive approach to heal bony defects. However, three-dimensional cell-scaffold constructs display uneven oxygen supply resulting in inhomogeneous tissue quality. We assessed different strategies to improve oxygen supply in vitro. Scaffolds with differing inner surface were seeded with preosteoblastic cells and cultivated either statically or in perfusion bioreactors. Oxygen concentration and pH were measured in the center of the scaffolds. An inductive feedback mechanism was build to increase bioreactor pump speed according to the oxygen concentrations measured within the scaffolds. While pH remained stable, oxygen concentration decreased significantly under static conditions within the cell-seeded scaffolds. Reducing the scaffolds' inner surface as well as increasing perfusion speeds in bioreactors resulted in improved oxygen supply. We conclude that improving oxygen supply to three dimensional culture systems for bone tissue engineering is feasible in an automated manner. Culture conditions have to be adapted to each cell-scaffold system individually.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.