ArticleJournal of industrial microbiology & biotechnology2012
Functional expression of trypsin from Streptomyces griseus by Pichia pastoris.
Article in Journal of industrial microbiology & biotechnology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 27 citations in OpenAlex.
- Improving production of Streptomyces griseus trypsin for enzymatic processing of insulin precursor.Microbial cell factories · 2020Article
- Production and cleavage of a fusion protein of porcine trypsinogen and enhanced green fluorescent protein (EGFP) in Pichia pastoris.Folia microbiologica · 2018Article
- Recent advances of molecular toolbox construction expand Pichia pastoris in synthetic biology applications.World journal of microbiology & biotechnology · 2017Review
- Functional inclusion bodies produced in the yeast Pichia pastoris.Microbial cell factories · 2016Article
- Improved Production of Active Streptomyces griseus Trypsin with a Novel Auto-Catalyzed Strategy.Scientific reports · 2016Article
- Biochemical Characterization of An Arginine-Specific Alkaline Trypsin from Bacillus licheniformis.International journal of molecular sciences · 2015Article
- Efficient secretion of inulin fructotransferase in Pichia pastoris using the formaldehyde dehydrogenase 1 promoter.Journal of industrial microbiology & biotechnology · 2014Article
- Improvement of catalytic efficiency and thermostability of recombinant Streptomyces griseus trypsin by introducing artificial peptide.World journal of microbiology & biotechnology · 2014Article
- Rational design of a novel propeptide for improving active production of Streptomyces griseus trypsin in Pichia pastoris.Applied and environmental microbiology · 2013Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the present study, the genes encoding trypsinogen and active trypsin from Streptomyces griseus were both cloned and expressed in the methylotrophic yeast Pichia pastoris with the α-factor secretion signal under the control of the alcohol oxidase promoter. The mature trypsin was successfully accumulated extracellularly in soluble form with a maximum amidase activity of 6.6 U ml(-1) (batch cultivation with flask cultivation) or 14.4 U ml(-1) (fed-batch cultivation with a 3-l fermentor). In contrast, the recombinant trypsinogen formed inclusion bodies and no activity was detected. Replacement of the trypsin propeptide Ala-Pro-Asn-Pro confirmed that its physiological function was as a repressor of activity. More importantly, our results proved that the propeptide inhibited the activity of trypsinogen after its successful folding.
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Registered trials
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