ArticleInflammation research : official journal of the European Histamine Research Society ... [et al.]2012
Characterization of lipopolysaccharide-stimulated cytokine expression in macrophages and monocytes.
Article in Inflammation research : official journal of the European Histamine Research Society ... [et al.], 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
- Attenuation of LPS-induced inflammatory responses in J774A.1 macrophages by phenylpropanoids and ursane triterpenes from Lavandula coronopifolia Poir.Scientific reports · 2026Article
- Molecular mechanisms behind the temporal dynamics of LPS-induced inflammatory response in splenic phagocytes of the wall lizard Hemidactylus flaviviridis.The Journal of experimental biology · 2026Article
- Cannabinoid receptor-2 ligand beta-caryophyllene modulates inflammatory responses of bovine peripheral blood mononuclear cells in vitro.Scientific reports · 2026Article
- M1 Macrophage-Derived Small Extracellular Vesicles as Synergistic Nanotherapeutics: Harnessing Intrinsic Anticancer Activity and Drug Delivery Capacity.Journal of extracellular vesicles · 2026Article
- Developing anACS pharmacology & translational science · 2025Article
- Targeting NF-κB Signaling: Selected Small Molecules Downregulate Pro-Inflammatory Cytokines in Both Food Allergen and LPS-Induced Inflammation.International journal of molecular sciences · 2024Article
- Altered inflammatory response in FMRP-deficient microglia.iScience · 2021Article
- In Vitro Effect of Replicated Porous Polymeric Nano-MicroStructured Biointerfaces Characteristics on Macrophages Behavior.Nanomaterials (Basel, Switzerland) · 2021Article
- Bone marrow deficiency of mRNA decaying protein Tristetraprolin increases inflammation and mitochondrial ROS but reduces hepatic lipoprotein production in LDLR knockout mice.Redox biology · 2020Article
- A functional role for eicosanoid-lysophospholipids in activating monocyte signaling.The Journal of biological chemistry · 2020Article
- Transcriptional profiling of human macrophages during infection withRNA biology · 2020Article
- TLR-2-mediated metabolic reprogramming participates in polyene phosphatidylcholine-mediated inhibition of M1 macrophage polarization.Immunologic research · 2020Article
- Direct electrical stimulation enhances osteogenesis by inducing Bmp2 and Spp1 expressions from macrophages and preosteoblasts.Biotechnology and bioengineering · 2019Article
- Salidroside Inhibits Lipopolysaccharide-ethanol-induced Activation of Proinflammatory Macrophages via Notch Signaling Pathway.Current medical science · 2019Article
- Developing a mouse model of acute encephalopathy using low-dose lipopolysaccharide injection and hyperthermia treatment.Experimental biology and medicine (Maywood, N.J.) · 2019Article
- Article
- Transcriptional and translational-uncoupling in regulation of the CXCL12 and its receptors CXCR4, 7 in THP-1 monocytes and macrophages.Immunity, inflammation and disease · 2018Article
- Elucidating the Role of CD84 and AHR in Modulation of LPS-Induced Cytokines Production by Cruciferous Vegetable-Derived Compounds Indole-3-Carbinol and 3,3'-Diindolylmethane.International journal of molecular sciences · 2018Article
- Polychlorinated Biphenyls (PCB 101, 153, and 180) Impair Murine Macrophage Responsiveness to Lipopolysaccharide: Involvement of NF-κB Pathway.Toxicological sciences : an official journal of the Society of Toxicology · 2015Article
- Temporal phenotypic features distinguish polarized macrophages in vitro.Autoimmunity · 2015Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveIn vitro cell culture models are widely used in inflammation research; however, information regarding the time- and dose-dependency of inflammatory responses toward LPS in these cell lines is scattered in the literature. MATERIAL: J774A.1 mouse macrophage and THP-1 human monocyte cell lines. TREATMENT: J774A.1 and THP-1 cells were treated with 0-500 ng/mL lipopolysaccharide for 0-24 h.
methodsSRB and BCA tests were used to measure total protein. Real-time PCR was used to determine gene expression levels, and ELISA was used to assess the protein levels. One-way ANOVA and Tukey's Honestly Significant Difference test were used to test the significance levels.
resultsIn J774A.1 and THP-1 cells, cytokines responded in distinct patterns upon LPS stimulation in a time- and dose-dependent manner, and the differential regulation of the response to LPS between J774A.1 and THP-1 cells appeared to correlate with the differential regulation of TLR4 at the mRNA level.
conclusionIn summary, this study indicated that temporal and dose-dependent responses to LPS need to be controlled for and that extrapolation of data on mechanisms may differ between cell lines of different origin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.