ArticleMolecular genetics and genomics : MGG2012
PhiC31 integrase-mediated cassette exchange in silkworm embryos.
Article in Molecular genetics and genomics : MGG, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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Who cites it
7 citing papers in PubMed, 18 citations in OpenAlex.
- An Effective Chemical Permeabilization of Silkworm Embryos.Bioengineering (Basel, Switzerland) · 2023Article
- SericinInsects · 2023Article
- A Bioengineering Approach for the Development of Fibroblast Growth Factor-7-Functionalized Sericin Biomaterial Applicable for the Cultivation of Keratinocytes.International journal of molecular sciences · 2022Article
- Genome engineering and parthenocloning in the silkworm, Bombyx mori.Journal of biosciences · 2015Review
- Construction of transformed, cultured silkworm cells and transgenic silkworm using the site-specific integrase system from phage φC31.Molecular biology reports · 2014Article
- New applications for phage integrases.Journal of molecular biology · 2014Review
- Efficient site-specific transgenesis and enhancer activity tests in medaka using PhiC31 integrase.Development (Cambridge, England) · 2013Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To construct an effective site-specific integration system in the silkworm, we examined if phiC31 integrase works in silkworm embryos. As an assay system, we constructed an extrachromosomal cassette exchange reaction system between two attP sites of an acceptor plasmid and two attB sites of a donor plasmid. To evaluate the activity, integrase mRNAs synthesized from three different plasmids were used. We injected a mixture of the acceptor and donor plasmids with the mRNA synthesized in vitro from one of the three plasmids into silkworm embryos at 4-6 h after oviposition and recovered plasmid DNAs from the embryos 3 days after injection. The resultant plasmids were transformed into Escherichia coli and spread on selection medium plates containing the appropriate antibiotics. A colony-forming assay and restriction enzyme digestion of the plasmids purified from the colonies showed that the phiC31 integrase worked very efficiently in the silkworm embryos. Notably, a phiC31 integrase mRNA synthesized from two of the plasmids produced cassette exchange plasmids at a high frequency, suggesting that the mRNA can be used to construct a targeted integration system in silkworms.
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Registered trials
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