ArticleBiochemical and biophysical research communications2012
Tyrosine phosphatase inhibition induces an ASC-dependent pyroptosis.
Article in Biochemical and biophysical research communications, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 17 citations in OpenAlex.
- cAbl Kinase Regulates Inflammasome Activation and Pyroptosis via ASC Phosphorylation.Journal of immunology (Baltimore, Md. : 1950) · 2021Article
- Stem Cell-Derived Exosomes Ameliorate Doxorubicin-Induced Muscle Toxicity through Counteracting Pyroptosis.Pharmaceuticals (Basel, Switzerland) · 2020Article
- The Role of Protein Tyrosine Phosphatases in Inflammasome Activation.International journal of molecular sciences · 2020Review
- Article
- Inflammasome Adaptor ASC Is Highly Elevated in Lung Over Plasma and Relates to Inflammation and Lung Diffusion in the Absence of Speck Formation.Frontiers in immunology · 2020Article
- Tyrosine Dephosphorylation of ASC Modulates the Activation of the NLRP3 and AIM2 Inflammasomes.Frontiers in immunology · 2019Article
- Recent Advances in the Molecular Mechanisms Underlying Pyroptosis in Sepsis.Mediators of inflammation · 2018Review
- Ethanol and Other Short-Chain Alcohols Inhibit NLRP3 Inflammasome Activation through Protein Tyrosine Phosphatase Stimulation.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- Article
- Article
- Inflammasome priming by lipopolysaccharide is dependent upon ERK signaling and proteasome function.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Pyroptosis is a type of cell death in which danger associated molecular patterns (DAMPs) and pathogen associated molecular patterns (PAMPs) induce mononuclear phagocytes to activate caspase-1 and release mature IL-1β. Because the tyrosine kinase inhibitor AG126 can prevent DAMP/PAMP induced activation of caspase-1, we hypothesized that tipping the tyrosine kinase/phosphatase balance toward phosphorylation would promote caspase-1 activation and cell death. THP-1 derived macrophages were therefore treated with the potent specific tyrosine phosphatase inhibitor, sodium orthovanadate (OVN) and analyzed for caspase-1 activation and cell death. OVN induced generalized increase in phosphorylated proteins, IL-1β release and cell death in a time and dose dependent pattern. This OVN induced pyroptosis correlated with speck formations that contained the apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC). Culturing the cells in the presence of extracellular K(+) (known to inhibit ATP dependent pyroptosis), a caspase inhibitor (ZVAD) or down regulating the expression of ASC with stable expression of siASC prevented the OVN induced pyroptosis. These data demonstrate that pyroptotic death is linked to tyrosine phosphatase activity providing novel targets for future pharmacologic interventions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.