ArticleGeneral and comparative endocrinology2012
Knockdown of leptin A expression dramatically alters zebrafish development.
Article in General and comparative endocrinology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 45 citations in OpenAlex.
- Genome-Wide Meta-Analysis Unravels Interactions between Magnesium Homeostasis and Metabolic Phenotypes.Journal of the American Society of Nephrology : JASN · 2018Pooled it
- Article
- Characterization of adaptive expression regulation of yellowtail kingfish (Seriola lalandi) leptin, receptor, and receptor overlapping transcript genes in response to fasting and re-feeding strategies.Fish physiology and biochemistry · 2024Article
- Tissue-Specific Effects of Leptin on Glucose and Lipid Metabolism.Endocrine reviews · 2021Review
- Endospanin Is a Candidate for Regulating Leptin Sensitivity.Frontiers in physiology · 2021Article
- Temporal expression profiles of leptin and its receptor genes during early development and ovarian maturation of Cynoglossus semilaevis.Fish physiology and biochemistry · 2020Article
- Article
- On the Molecular Evolution of Leptin, Leptin Receptor, and Endospanin.Frontiers in endocrinology · 2017Article
- Comparative Physiology of Energy Metabolism: Fishing for Endocrine Signals in the Early Vertebrate Pool.Frontiers in endocrinology · 2017Review
- Mechanisms of otoconia and otolith development.Developmental dynamics : an official publication of the American Association of Anatomists · 2015Review
- The impact of leptin on perinatal development and psychopathology.Journal of chemical neuroanatomy · 2014Review
- Leptin and IL-6 family cytokines synergize to stimulate Müller glia reprogramming and retina regeneration.Cell reports · 2014Article
- Comparative endocrinology of leptin: assessing function in a phylogenetic context.General and comparative endocrinology · 2014Review
- Discovery of the elusive leptin in birds: identification of several 'missing links' in the evolution of leptin and its receptor.PloS one · 2014Article
- Leptin expression affects metabolic rate in zebrafish embryos (D. rerio).Frontiers in physiology · 2013Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
Abstract
Using morpholino antisense oligonucleotide (MO) technology, we blocked leptin A or leptin receptor expression in embryonic zebrafish, and analyzed consequences of leptin A knock-down on fish development. Embryos injected with leptin A or leptin receptor MOs (leptin A or leptin receptor morphants) had smaller bodies and eyes, undeveloped inner ear, enlarged pericardial cavity, curved body and/or tail and larger yolk compared to control embryos of the same stages. The defects persisted in 6-9 days old larvae. We found that blocking leptin A function had little effect on the development of early brain (1 day old), but differentiation of both the morphant dorsal brain and retinal cells was severely disrupted in older (2 days old) embryos. Despite the enlarged pericardial cavity, differentiation of cardiac cells appeared to be similar to control embryos. Formation of the morphants' inner ear is also severely disrupted, which corroborates existing reports of leptin receptor expression in inner ear of both zebrafish and mammals. Co-injection of leptin A MO and recombinant leptin results in partial rescue of the wild-type phenotype. Our results suggest that leptin A plays distinct roles in zebrafish development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.