ArticleDevelopmental biology2012
Activation of germline-specific genes is required for limb regeneration in the Mexican axolotl.
Article in Developmental biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Article
- Sex-biased gene expression precedes sexual dimorphism in the agonadal annelid Platynereis dumerilii.Development (Cambridge, England) · 2025Article
- Sex-biased gene expression precedes sexual dimorphism in the agonadal annelidbioRxiv : the preprint server for biology · 2024Article
- The salamander blastema within the broader context of metazoan regeneration.Frontiers in cell and developmental biology · 2023Review
- Transposon control as a checkpoint for tissue regeneration.Development (Cambridge, England) · 2022Article
- Rebuilding limbs, one cell at a time.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Article
- A pan-metazoan concept for adult stem cells: the wobbling Penrose landscape.Biological reviews of the Cambridge Philosophical Society · 2022Article
- Post-amputation reactive oxygen species production is necessary for axolotls limb regeneration.Frontiers in cell and developmental biology · 2022Article
- PIWI-mediated control of tissue-specific transposons is essential for somatic cell differentiation.Cell reports · 2021Article
- Review
- Review
- Expression ofGenes · 2020Article
- Site-Specific Expression Pattern of PIWI-Interacting RNA in Skin and Oral Mucosal Wound Healing.International journal of molecular sciences · 2020Article
- Parallels between wound healing, epimorphic regeneration and solid tumors.Development (Cambridge, England) · 2020Review
- Characterization of the piRNA pathway during development of the sea anemone Nematostella vectensis.RNA biology · 2017Article
- Piwi Is Required to Limit Exhaustion of Aging Somatic Stem Cells.Cell reports · 2017Article
- The Axolotl Limb Regeneration Model as a Discovery Tool for Engineering the Stem Cell Niche.Current stem cell reports · 2017Article
- Advances in Decoding Axolotl Limb Regeneration.Trends in genetics : TIG · 2017Review
- A Tissue-Mapped Axolotl De Novo Transcriptome Enables Identification of Limb Regeneration Factors.Cell reports · 2017Article
- Article
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Authors and funding
10 authors.
Funding
Abstract
The capacity for tissue and organ regeneration in humans is dwarfed by comparison to that of salamanders. Emerging evidence suggests that mechanisms learned from the early phase of salamander limb regeneration-wound healing, cellular dedifferentiation and blastemal formation-will reveal therapeutic approaches for tissue regeneration in humans. Here we describe a unique transcriptional fingerprint of regenerating limb tissue in the Mexican axolotl (Ambystoma mexicanum) that is indicative of cellular reprogramming of differentiated cells to a germline-like state. Two genes that are required for self-renewal of germ cells in mice and flies, Piwi-like 1 (PL1) and Piwi-like 2 (PL2), are expressed in limb blastemal cells, the basal layer keratinocytes and the thickened apical epithelial cap in the wound epidermis in the regenerating limb. Depletion of PL1 and PL2 by morpholino oligonucleotides decreased cell proliferation and increased cell death in the blastema leading to a significant retardation of regeneration. Examination of key molecules that are known to be required for limb development or regeneration further revealed that FGF8 is transcriptionally downregulated in the presence of the morpholino oligos, indicating PL1 and PL2 might participate in FGF signaling during limb regeneration. Given the requirement for FGF signaling in limb development and regeneration, the results suggest that PL1 and PL2 function to establish a unique germline-like state that is associated with successful regeneration.
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