ArticleMethods (San Diego, Calif.)2012
Studying protein complexes by the yeast two-hybrid system.
Article in Methods (San Diego, Calif.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Combined proximity labeling and affinity purification-mass spectrometry workflow for mapping and visualizing protein interaction networks.Nature protocols · 2020Article
- Network of Interactions between ZIKA Virus Non-Structural Proteins and Human Host Proteins.Cells · 2020Article
- Resurrected Protein Interaction Networks Reveal the Innovation Potential of Ancient Whole-Genome Duplication.The Plant cell · 2018Article
- Integrative proteomics to understand the transmission mechanism of Barley yellow dwarf virus-GPV by its insect vector Rhopalosiphum padi.Scientific reports · 2015Article
- Article
- Accurate protein complex retrieval by affinity enrichment mass spectrometry (AE-MS) rather than affinity purification mass spectrometry (AP-MS).Molecular & cellular proteomics : MCP · 2015Article
- Mapping the Protein-Protein Interactome Networks Using Yeast Two-Hybrid Screens.Advances in experimental medicine and biology · 2015Review
- Detecting protein-protein interactions based on kinase-mediated growth induction of mammalian cells.Scientific reports · 2014Article
- A comprehensive protein-protein interactome for yeast PAS kinase 1 reveals direct inhibition of respiration through the phosphorylation of Cbf1.Molecular biology of the cell · 2014Article
- The binary protein-protein interaction landscape of Escherichia coli.Nature biotechnology · 2014Article
- Systems biology in the context of big data and networks.BioMed research international · 2014Review
- Article
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Authors and funding
5 authors.
Funding
Abstract
Protein complexes are typically analyzed by affinity purification and subsequent mass spectrometric analysis. However, in most cases the structure and topology of the complexes remains elusive from such studies. Here we investigate how the yeast two-hybrid system can be used to analyze direct interactions among proteins in a complex. First we tested all pairwise interactions among the seven proteins of Escherichia coli DNA polymerase III as well as an uncharacterized complex that includes MntR and PerR. Four and seven interactions were identified in these two complexes, respectively. In addition, we review Y2H data for three other complexes of known structure which serve as "gold-standards", namely Varicella Zoster Virus (VZV) ribonucleotide reductase (RNR), the yeast proteasome, and bacteriophage lambda. Finally, we review an Y2H analysis of the human spliceosome which may serve as an example for a dynamic mega-complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.