ArticleTissue engineering. Part A2013
Live-cell, temporal gene expression analysis of osteogenic differentiation in adipose-derived stem cells.
Article in Tissue engineering. Part A, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 8 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed, 31 citations in OpenAlex.
- Decontamination of Titanium Surface Using Different Methods: An In Vitro Study.Materials (Basel, Switzerland) · 2020Article
- Bone morphogenetic protein 9 (BMP9) induces effective bone formation from reversibly immortalized multipotent adipose-derived (iMAD) mesenchymal stem cells.American journal of translational research · 2016Article
- Single-cell detection of mRNA expression using nanofountain-probe electroporated molecular beacons.Small (Weinheim an der Bergstrasse, Germany) · 2015Article
- Live-cell, temporal gene expression analysis of osteogenic differentiation in adipose-derived stem cells.Tissue engineering. Part A · 2014Article
- Retraction of Desai, et al. Tissue Engineering, Part A, 19;1/2:40-48.Tissue engineering. Part A · 2014Article
- Tracking neuronal marker expression inside living differentiating cells using molecular beacons.Frontiers in cellular neuroscience · 2013Article
- Deconstructing stem cell population heterogeneity: single-cell analysis and modeling approaches.Biotechnology advances · 2013Review
- SOX2 and OCT4 mRNA-expressing cells, detected by molecular beacons, localize to the center of neurospheres during differentiation.PloS one · 2013Article
Corrections and comments
- Retraction · 2014-02-28Error in Image · Error in Methods ·
- Retracted
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Adipose-derived stem cells (ASCs) are a widely investigated type of mesenchymal stem cell with great potential for musculoskeletal regeneration. However, use of ASCs is complicated by their cellular heterogeneity, which exists at both the population and single-cell levels. This study demonstrates a live-cell assay to investigate gene expression in ASCs undergoing osteogenesis using fluorescently tagged DNA hybridization probes called molecular beacons. Three molecular beacons were designed to target mRNA sequences for alkaline phosphatase, type I collagen, and osteocalcin (ALPL, COL1A1, and BGLAP), genes characteristically expressed during osteogenesis. The percentage of cells expressing these genes in a population was monitored daily to quantify the uniformity of the differentiation process. Differentiating ASC populations were repeatedly measured in a nondestructive fashion over a 21-day period to obtain temporal gene expression data. Results showed consistent expression patterns for the investigated osteogenic genes in response to induction medium. Peak expression was observed at days 3-4 for ALPL, day 14 for COL1A1, and day 21 for BGLAP. Additionally, the differentiation response of sample populations became more uniform after 2 weeks in osteogenic induction medium, suggesting a syncing of ASCs occurs over time. These findings are consistent with previous studies of osteogenic differentiation and suggest that molecular beacons are a viable means to monitor the spatiotemporal gene expression of live, differentiating ASCs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.