ArticleVeterinary research2012
VtaA8 and VtaA9 from Haemophilus parasuis delay phagocytosis by alveolar macrophages.
Article in Veterinary research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 64 citations in OpenAlex.
- A novel function of RHOA as a host-dependent factor in Glaesserella parasuis infection of LLC-PK1 cells.Veterinary research · 2025Article
- Pathotyping and molecular serotyping of clinical isolates of Glaesserella parasuis in Taiwan.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Genomic insights into diversity, antimicrobial resistance and virulence ofFrontiers in microbiology · 2025Article
- Virulence and Antimicrobial Resistance Characterization ofAntibiotics (Basel, Switzerland) · 2024Article
- Molecular characterization of Glaesserella parasuis strains circulating in North American swine production systems.BMC veterinary research · 2023Article
- Immune responses following neonatal vaccination with conserved F4 fragment of VtaA proteins from virulentVaccine: X · 2023Article
- Pan-genome wide association study ofFrontiers in microbiology · 2023Article
- TurboID Screening of the OmpP2 Protein Reveals Host Proteins Involved in Recognition and Phagocytosis ofMicrobiology spectrum · 2022Article
- Dosing Regimen of Aditoprim and Sulfamethoxazole Combination for thePharmaceutics · 2022Article
- Temporal Patterns of Phenotypic Antimicrobial Resistance and Coinfecting Pathogens inPathogens (Basel, Switzerland) · 2022Article
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- Characterization and protective activity of monoclonal antibodies directed against Fe (3+) ABC transporter substrate-binding protein of Glaesserella parasuis.Veterinary research · 2021Article
- Sow Vaccination with a Protein Fragment against VirulentVaccines · 2021Article
- Molecular characterization of Glaesserella parasuis strains isolated from North America, Europe and Asia by serotyping PCR and LS-PCR.Veterinary research · 2021Article
- The Role of Antibodies Against the Crude Capsular Extract in the Immune Response of Porcine Alveolar Macrophages toFrontiers in immunology · 2021Article
- Proteome Analysis of Outer Membrane Vesicles From a Highly Virulent Strain ofFrontiers in veterinary science · 2021Article
- Serotyping and pathotyping of Glaesserella parasuis isolated 2012-2019 in Germany comparing different PCR-based methods.Veterinary research · 2020Article
- Haemophilus parasuis VtaA2 is involved in adhesion to extracellular proteins.Veterinary research · 2019Article
- Comparative genomic and methylome analysis of non-virulent D74 and virulent Nagasaki Haemophilus parasuis isolates.PloS one · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Haemophilus parasuis, a member of the family Pasteurellaceae, is a common inhabitant of the upper respiratory tract of healthy pigs and the etiological agent of Glässer's disease. As other virulent Pasteurellaceae, H. parasuis can prevent phagocytosis, but the bacterial factors involved in this virulence mechanism are not known. In order to identify genes involved in phagocytosis resistance, we constructed a genomic library of the highly virulent reference strain Nagasaki and clones were selected by increased survival after incubation with porcine alveolar macrophages (PAM). Two clones containing two virulent-associated trimeric autotransporter (VtaA) genes, vtaA8 and vtaA9, respectively, were selected by this method. A reduction in the interaction of the two clones with the macrophages was detected by flow cytometry. Monoclonal antibodies were produced and used to demonstrate the presence of these proteins on the bacterial surface of the corresponding clone, and on the H. parasuis phagocytosis-resistant strain PC4-6P. The effect of VtaA8 and VtaA9 in the trafficking of the bacteria through the endocytic pathway was examined by fluorescence microscopy and a delay was detected in the localization of the vtaA8 and vtaA9 clones in acidic compartments. These results are compatible with a partial inhibition of the routing of the bacteria via the degradative phagosome. Finally, antibodies against a common epitope in VtaA8 and VtaA9 were opsonic and promoted phagocytosis of the phagocytosis-resistant strain PC4-6P by PAM. Taken together, these results indicate that VtaA8 and VtaA9 are surface proteins that play a role in phagocytosis resistance of H. parasuis.
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