ArticlePlant methods2012
A high throughput DNA extraction method with high yield and quality.
Article in Plant methods, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed.
- A Dual-gRNA CRISPR/Cas9 System for Efficient Generation of Large Fragment Deletions in Poplar.Bio-protocol · 2026Article
- Comparative genome analysis identifies necrosis-inducing effectors in the lentil pathogen Ascochyta lentis in isolates with distinct virulence profiles.BMC genomics · 2026Article
- A key heavy metal-binding protein orchestrates plant resistance against a geminivirus.Fundamental research · 2026Article
- Joint test of historical vs. contemporary biogeography supports abundant center hypothesis shaping spatial patterns of self-fertilization.Evolution letters · 2026Article
- Analysing fungal microbiome differences between the roots of healthy and diseased Chinese hickory (Carya cathayensis) trees.Scientific reports · 2025Article
- An alpine plant shows no decrease in genetic diversity associated with rapid post-glacial range expansion.American journal of botany · 2025Article
- Effects of a novel loss-of-function waxy endosperm allele on sorghum seed development and grain quality.G3 (Bethesda, Md.) · 2025Article
- Construction of a Genome-Wide Copy Number Variation Map and Association Analysis of Black Spot in Jujube.Plants (Basel, Switzerland) · 2025Article
- Comparative evaluation of various DNA extraction methods and analysis of DNA degradation levels in commercially marketed Chestnut rose juices and beverages.BMC biotechnology · 2025Article
- Repeat-induced point mutations driving Parastagonospora nodorum genomic diversity are balanced by selection against non-synonymous mutations.Communications biology · 2024Article
- High-quality Chromosomal-Level Genome Assembly of the Wasabi (Eutrema japonicum) 'Magic'.Scientific data · 2024Article
- Genetic analysis of yield components in buckwheat using high-throughput sequencing analysis and wild resource populations.Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- A haplotype-resolved chromosome-level assembly and annotation of European hazelnut (C. avellana cv. Jefferson) provides insight into mechanisms of eastern filbert blight resistance.G3 (Bethesda, Md.) · 2024Article
- The C4 Protein of TbLCYnV Promotes SnRK1 β2 Degradation Via the Autophagy Pathway to Enhance Viral Infection inViruses · 2024Article
- Crop DNA extraction with lab-made magnetic nanoparticles.PloS one · 2024Article
- Article
- Bacterial Microbiome Differences between the Roots of Diseased and Healthy Chinese Hickory (Journal of microbiology and biotechnology · 2023Article
- Article
- Comparative genomics of smut fungi suggest the ability of meiosis and mating in asexual species of the genus Pseudozyma (Ustilaginales).BMC genomics · 2023Article
- Haploidy and aneuploidy in switchgrass mediated by misexpression of CENH3.The plant genome · 2023Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPreparation of large quantity and high quality genomic DNA from a large number of plant samples is a major bottleneck for most genetic and genomic analyses, such as, genetic mapping, TILLING (Targeting Induced Local Lesion IN Genome), and next-generation sequencing directly from sheared genomic DNA. A variety of DNA preparation methods and commercial kits are available. However, they are either low throughput, low yield, or costly. Here, we describe a method for high throughput genomic DNA isolation from sorghum [Sorghum bicolor (L.) Moench] leaves and dry seeds with high yield, high quality, and affordable cost.
resultsWe developed a high throughput DNA isolation method by combining a high yield CTAB extraction method with an improved cleanup procedure based on MagAttract kit. The method yielded large quantity and high quality DNA from both lyophilized sorghum leaves and dry seeds. The DNA yield was improved by nearly 30 fold with 4 times less consumption of MagAttract beads. The method can also be used in other plant species, including cotton leaves and pine needles.
conclusionA high throughput system for DNA extraction from sorghum leaves and seeds was developed and validated. The main advantages of the method are low cost, high yield, high quality, and high throughput. One person can process two 96-well plates in a working day at a cost of $0.10 per sample of magnetic beads plus other consumables that other methods will also need.
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