ArticleThe Biochemical journal2012
Dedifferentiated follicular granulosa cells derived from pig ovary can transdifferentiate into osteoblasts.
Article in The Biochemical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- The Effect of Liposomal DMU-212 on the Differentiation of Human Ovarian Granulosa Cells in a Primary 3D Culture Model.Pharmaceuticals (Basel, Switzerland) · 2025Article
- The Molecular Quality and Mitochondrial Activity of Porcine Cumulus-Oocyte Complexes Are Affected by Their Exposure to Three Endocrine-Active Compounds under 3D In Vitro Maturation Conditions.International journal of molecular sciences · 2022Article
- Transcriptomic Profile of New Gene Markers Encoding Proteins Responsible for Structure of Porcine Ovarian Granulosa Cells.Biology · 2021Article
- Characterization of stem cells from human ovarian follicular fluid; a potential source of autologous stem cell for cell-based therapy.Human cell · 2021Article
- Characteristics of Circular RNA Expression Profiles of Porcine Granulosa Cells in Healthy and Atretic Antral Follicles.International journal of molecular sciences · 2020Article
- Review
- Interaction between growing oocytes and granulosa cells in vitro.Reproductive medicine and biology · 2020Review
- Evaluation of stemness marker expression in bovine ovarian granulosa cells.Animal reproduction · 2019Article
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Morphological changes during replicative senescence in bovine ovarian granulosa cells.Cell cycle (Georgetown, Tex.) · 2019Article
- 'Heart development and morphogenesis' is a novel pathway for human ovarian granulosa cell differentiation during long‑term in vitro cultivation‑a microarray approach.Molecular medicine reports · 2019Article
- Genes responsible for proliferation, differentiation, and junction adhesion are significantly up-regulated in human ovarian granulosa cells during a long-term primary in vitro culture.Histochemistry and cell biology · 2019Article
- Ovarian regeneration: The potential for stem cell contribution in the postnatal ovary to sustained endocrine function.Molecular and cellular endocrinology · 2017Review
- Potential differentiation ability of gingiva originated human mesenchymal stem cell in the presence of tacrolimus.Scientific reports · 2016Article
- Efficient differentiation of steroidogenic and germ-like cells from epigenetically-related iPSCs derived from ovarian granulosa cells.PloS one · 2015Article
- Expression of mesenchymal stem cells-related genes and plasticity of aspirated follicular cells obtained from infertile women.BioMed research international · 2014Article
- Plasticity of granulosa cells: on the crossroad of stemness and transdifferentiation potential.Journal of assisted reproduction and genetics · 2013Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transdifferentiation is the conversion of cells from one differentiated cell type into another. How functionally differentiated cells already committed to a specific cell lineage can transdifferentiate into other cell types is a key question in cell biology and regenerative medicine. In the present study we show that porcine ovarian follicular GCs (granulosa cells) can transdifferentiate into osteoblasts in vitro and in vivo. Pure GCs isolated and cultured in Dulbecco's modified Eagle's medium supplemented with 20% FBS (fetal bovine serum) proliferated and dedifferentiated into fibroblast-like cells. We referred to these cells as DFOG (dedifferentiated follicular granulosa) cells. Microarray analysis showed that DFOG cells lost expression of GC-specific marker genes, but gained the expression of osteogenic marker genes during dedifferentiation. After osteogenic induction, DFOG cells underwent terminal osteoblast differentiation and matrix mineralization in vitro. Furthermore, when DFOG cells were transplanted subcutaneously into SCID mice, these cells formed ectopic osteoid tissue. These results indicate that DFOG cells derived from GCs can differentiate into osteoblasts in vitro and in vivo. We suggest that GCs provide a useful model for studying the mechanisms of transdifferentiation into other cell lineages in functionally differentiated cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.