ArticleJournal of advanced pharmaceutical technology & research2012
Development and validation of a HPLC method for the determination of trans-resveratrol in spiked human plasma.
Article in Journal of advanced pharmaceutical technology & research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 48 citations in OpenAlex.
- Development of a lozenge for oral transmucosal delivery of trans-resveratrol in humans: proof of concept.PloS one · 2014Trial
- Curcumin and EGCG combined formulation in nanostructured lipid carriers for anti-aging applications.International journal of pharmaceutics: X · 2025Article
- Analytical Techniques for the Quantification and Validation of Resveratrol: A Review.Anti-inflammatory & anti-allergy agents in medicinal chemistry · 2025Review
- Article
- Dual combination of resveratrol and pterostilbene aqueous core nanocapsules for integrated prostate cancer targeting.Therapeutic delivery · 2024Article
- Quality by Design (QbD) Based Method for Estimation of Xanthohumol in Bulk and Solid Lipid Nanoparticles and Validation.Molecules (Basel, Switzerland) · 2023Article
- Quality by Design Based Formulation of Xanthohumol Loaded Solid Lipid Nanoparticles with Improved Bioavailability and Anticancer Effect against PC-3 Cells.Pharmaceutics · 2022Article
- Donepezil HCl Liposomes: Development, Characterization, Cytotoxicity, and Pharmacokinetic Study.AAPS PharmSciTech · 2022Article
- Liquid crystalline assembly for potential combinatorial chemo-herbal drug delivery to lung cancer cells.International journal of nanomedicine · 2019Article
- Rapid quantification of resveratrol in mouse plasma by ultra high pressure liquid chromatography (UPLC) coupled to tandem mass spectrometry.Puerto Rico health sciences journal · 2014Article
- Development and evaluation of 6-mercaptopurine and metoclopramide polypill formulation for oral administration: In-vitro and ex vivo studies.International journal of pharmaceutical investigation · 2013Article
- Quantitative analysis of Glycyrrhizic acid from a polyherbal preparation using liquid chromatographic technique.Journal of advanced pharmaceutical technology & research · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A simple, accurate, precise, sensitive, and reproducible high-performance liquid chromatography method was developed for the determination of Resveratrol (trans-3, 4',5-trihydroxystilbene) in human plasma using liquid-liquid extraction. Caffeine was employed as an internal standard (IS). However, little information is known about its distribution in the organism generally because of the lack of accurate and precise detection methods. The chromatographic separation was achieved on a Phenomenex C18 column (250 mm × 4.6 mm, 5 μm) at room temperature in isocratic mode, and the column effluent was monitored by UV detector at 306 nm. The mobile phase used was methanol: phosphate buffer (pH 6.8 adjusted with 0.5% (v/v) orthophosphoric acid solution in Milli-Q water) (63:37%, v/v) at a flow rate of 1.0 ml/min. Nominal retention times of trans-resveratrol and IS were 3.94 and 7.86 minutes, respectively. Limits of detection and Limits of quantification of trans-resveratrol were 0.006 μg/ml and 0.008 μg/ml, respectively. This method was linear over the range of 0.010 to 6.4 μg/ml with regression coefficient greater than 0.9998. The inter- and intra-day precisions in the samples, 0.010, 3.2 and 6.4 μg/ml of trans-resveratrol was in the range 0.63 to 2.12% relative standard deviation (RSD) and 0.46 to 1.02% RSD, respectively. Resveratrol was found to be stable for a period of 15 days on storage at -20°C. The method was found to be precise, accurate, and specific during the study.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.