ArticleJournal of clinical microbiology2012
Real-time PCR for diagnosing Helicobacter pylori infection in patients with upper gastrointestinal bleeding: comparison with other classical diagnostic methods.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 66 citations in OpenAlex.
- Non-invasive diagnostic tests for Helicobacter pylori infection.The Cochrane database of systematic reviews · 2018Pooled it
- Update on the pathogenesis and clinical management ofWorld journal of gastrointestinal pathophysiology · 2025Review
- Comparative gastric microbiota profiles in non-ulcer dyspepsia and peptic ulcer patients.BMC microbiology · 2025Article
- Review
- Multiple cross displacement amplification-based lateral flow biosensor for rapid and sensitive detection ofFrontiers in cellular and infection microbiology · 2024Article
- New CagL Amino Acid Polymorphism Patterns ofMedicina (Kaunas, Lithuania) · 2022Article
- Helicobacter pylori Infection, Its Laboratory Diagnosis, and Antimicrobial Resistance: a Perspective of Clinical Relevance.Clinical microbiology reviews · 2022Review
- Rapid and Sensitive Assay ofFrontiers in microbiology · 2022Article
- Efficacy of immunohistochemical staining in detecting <em>Helicobacter pylori</em> in Saudi patients with minimal and atypical infection.European journal of histochemistry : EJH · 2021Article
- Development and Validation of Multiplex Quantitative PCR Assay for Detection ofInfection and drug resistance · 2021Article
- Article
- Accuracy of theTherapeutic advances in gastroenterology · 2020Review
- The Inhibitory Effect of Mouse Gastric DNA on Amplification of Helicobacter pylori Genomic DNA in Quantitative PCRIranian biomedical journal · 2019Article
- Detection ofRSC advances · 2018Article
- Gastric Juice-Based Real-Time PCR for TailoredInternational journal of medical sciences · 2017Article
- Braf, Kras and Helicobacter pylori epigenetic changes-associated chronic gastritis in Egyptian patients with and without gastric cancer.World journal of microbiology & biotechnology · 2016Article
- Diagnosis of Helicobacter pylori infection: Current options and developments.World journal of gastroenterology · 2015Review
- Relationship between tobacco, cagA and vacA i1 virulence factors and bacterial load in patients infected by Helicobacter pylori.PloS one · 2015Article
- Diagnosis of Helicobacter pylori: what should be the gold standard?World journal of gastroenterology · 2014Review
- Helicobacter pylori infection - recent developments in diagnosis.World journal of gastroenterology · 2014Review
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to determine the diagnostic usefulness of quantification of the H. pylori genome in detection of infection in patients with upper gastrointestinal bleeding (UGB). A total of 158 consecutive patients with digestive disorders, 80 of whom had clinical presentation of UGB, were studied. The number of microorganisms was quantified using a real-time PCR system which amplifies the urease gene with an internal control for eliminating the false negatives. A biopsy sample from the antrum and corpus of each patient was processed. The rapid urease test, culture, histological study, stool antigen test, and breath test were done. The gold standard was a positive culture or positive results in at least two of the other techniques. When a positive result was defined as any number of microorganisms/human cell, the sensitivity of real-time PCR was greater in bleeding patients, especially in the gastric corpus: 68.4% (95% confidence interval [CI], 52.3 to 84.5%) in non-UGB patients versus 91.5% (95% CI, 79.6 to 97.6%) in UGB patients. When a positive result was defined as a number of microorganisms/human cell above the optimal value that maximizes the Youden index (>3.56 microorganisms/human cell in the antrum and >2.69 in the corpus), the sensitivity and specificity in UGB patients were over 80% in both antrum and corpus. Our findings suggest that some bleeding patients with infection caused by H. pylori may not be correctly diagnosed by classical methods, and such patients could benefit from the improved diagnosis provided by real-time PCR. However, the clinical significance of a small number of microorganisms in patients with negative results in classical tests should be evaluated.
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Registered trials
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