ArticlePlanta2012
Molecular characterization of the pentacyclic triterpenoid biosynthetic pathway in Catharanthus roseus.
Article in Planta, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 136 citations in OpenAlex.
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- Combinatorial optimization and spatial remodeling of CYPs to control product profile.Metabolic engineering · 2023Article
- Hydroxylases involved in terpenoid biosynthesis: a review.Bioresources and bioprocessing · 2023Review
- Squalene hopene cyclases and oxido squalene cyclases: potential targets for regulating cyclisation reactions.Biotechnology letters · 2023Review
- Isolation of two triterpenoids fromFrontiers in plant science · 2023Article
- Transcriptome Analyses Revealed the Key Metabolic Genes and Transcription Factors Involved in Terpenoid Biosynthesis in Sacred Lotus.Molecules (Basel, Switzerland) · 2022Article
- Cytochrome P450 monooxygenase-mediated tailoring of triterpenoids and steroids in plants.Beilstein journal of organic chemistry · 2022Review
- Genome-Wide Identification of OSC Gene Family and Potential Function in the Synthesis of Ursane- and Oleanane-Type Triterpene inInternational journal of molecular sciences · 2021Article
- The Euscaphis japonica genome and the evolution of malvids.The Plant journal : for cell and molecular biology · 2021Article
- A Review on Preparation of Betulinic Acid and Its Biological Activities.Molecules (Basel, Switzerland) · 2021Review
- Tumor-suppressing effect of bartogenic acid in ovarian (SKOV-3) xenograft mouse model.Naunyn-Schmiedeberg's archives of pharmacology · 2021Article
- Ginsenosides inActa pharmaceutica Sinica. B · 2021Review
- Biocatalysis in the Chemistry of Lupane Triterpenoids.Molecules (Basel, Switzerland) · 2021Review
- Rational design strategies for functional reconstitution of plant cytochrome P450s in microbial systems.Current opinion in plant biology · 2021Review
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- Osmolytes and membrane lipids in adaptive response of thermophilic fungus Rhizomucor miehei to cold, osmotic and oxidative shocks.Extremophiles : life under extreme conditions · 2020Article
- Biotechnological production of betulinic acid and derivatives and their applications.Applied microbiology and biotechnology · 2020Review
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Catharanthus roseus is an important medicinal plant and the sole commercial source of monoterpenoid indole alkaloids (MIA), anticancer compounds. Recently, triterpenoids like ursolic acid and oleanolic acid have also been found in considerable amounts in C. roseus leaf cuticular wax layer. These simple pentacyclic triterpenoids exhibit various pharmacological activities such as anti-inflammatory, anti-tumor and anti-microbial properties. Using the EST collection from C. roseus leaf epidermome ( http://www.ncbi.nlm.nih.gov/dbEST ), we have successfully isolated a cDNA (CrAS) encoding 2,3-oxidosqualene cyclase (OSC) and a cDNA (CrAO) encoding amyrin C-28 oxidase from the leaves of C. roseus. The functions of CrAS and CrAO were analyzed in yeast (Saccharomyces cerevisiae) systems. CrAS was characterized as a novel multifunctional OSC producing α- and β-amyrin in a ratio of 2.5:1, whereas CrAO was a multifunctional C-28 oxidase converting α-amyrin, β-amyrin and lupeol to ursolic-, oleanolic- and betulinic acids, respectively, via a successive oxidation at the C-28 position of the substrates. In yeast co-expressing CrAO and CrAS, ursolic- and oleanolic acids were detected in the yeast cell extracts, while the yeast cells co-expressing CrAO and AtLUP1 from Arabidopsis thaliana produced betulinic acid. Both CrAS and CrAO genes show a high expression level in the leaf, which was consistent with the accumulation patterns of ursolic- and oleanolic acids in C. roseus. These results suggest that CrAS and CrAO are involved in the pentacyclic triterpene biosynthesis in C. roseus.
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