Trial reportJournal of bioenergetics and biomembranes2012
Metabolic control analysis of cellular respiration in situ in intraoperational samples of human breast cancer.
Trial report in Journal of bioenergetics and biomembranes, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 49 citations in OpenAlex.
- Energy Metabolism Behavior and Response to Microenvironmental Factors of the Experimental Cancer Cell Models Differ from that of Actual Human Tumors.Mini reviews in medicinal chemistry · 2025Review
- Controlled power: how biology manages succinate-driven energy release.Biochemical Society transactions · 2021Article
- Curcumin and Carnosic Acid Cooperate to Inhibit Proliferation and Alter Mitochondrial Function of Metastatic Prostate Cancer Cells.Antioxidants (Basel, Switzerland) · 2021Article
- Molecular and Supramolecular Structure of the Mitochondrial Oxidative Phosphorylation System: Implications for Pathology.Life (Basel, Switzerland) · 2021Review
- Energy Metabolic Plasticity of Colorectal Cancer Cells as a Determinant of Tumor Growth and Metastasis.Frontiers in oncology · 2021Review
- Mitochondrial Respiration inCancers · 2020Article
- Metabolic and OXPHOS Activities Quantified by TemporalFrontiers in oncology · 2020Article
- Adenylate Kinase and Metabolic Signaling in Cancer Cells.Frontiers in oncology · 2020Review
- Reverse Warburg Effect-Related Mitochondrial Activity andNuclear medicine and molecular imaging · 2019Article
- Tubulin βII and βIII Isoforms as the Regulators of VDAC Channel Permeability in Health and Disease.Cells · 2019Review
- The complexity of mitochondrial outer membrane permeability and VDAC regulation by associated proteins.Journal of bioenergetics and biomembranes · 2018Article
- Review
- The reverse Warburg effect is likely to be an Achilles' heel of cancer that can be exploited for cancer therapy.Oncotarget · 2017Review
- AG311, a small molecule inhibitor of complex I and hypoxia-induced HIF-1α stabilization.Cancer letters · 2017Article
- Mitochondrial Respiration in Human Colorectal and Breast Cancer Clinical Material Is Regulated Differently.Oxidative medicine and cellular longevity · 2017Article
- Simple oxygraphic analysis for the presence of adenylate kinase 1 and 2 in normal and tumor cells.Journal of bioenergetics and biomembranes · 2016Article
- The Swi3 protein plays a unique role in regulating respiration in eukaryotes.Bioscience reports · 2016Article
- Metabolic remodeling in human colorectal cancer and surrounding tissues: alterations in regulation of mitochondrial respiration and metabolic fluxes.Biochemistry and biophysics reports · 2015Article
- Tumor microenvironment promotes dicarboxylic acid carrier-mediated transport of succinate to fuel prostate cancer mitochondria.American journal of cancer research · 2015Article
- The role of tubulin in the mitochondrial metabolism and arrangement in muscle cells.Journal of bioenergetics and biomembranes · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to analyze quantitatively cellular respiration in intraoperational tissue samples taken from human breast cancer (BC) patients. We used oxygraphy and the permeabilized cell techniques in combination with Metabolic Control Analysis (MCA) to measure a corresponding flux control coefficient (FCC). The activity of all components of ATP synthasome, and respiratory chain complexes was found to be significantly increased in human BC cells in situ as compared to the adjacent control tissue. FCC(s) were determined upon direct activation of respiration with exogenously-added ADP and by titrating the complexes with their specific inhibitors to stepwise decrease their activity. MCA showed very high sensitivity of all complexes and carriers studied in human BC cells to inhibition as compared to mitochondria in normal oxidative tissues. The sum of FCC(s) for all ATP synthasome and respiratory chain components was found to be around 4, and the value exceeded significantly that for normal tissue (close to 1). In BC cells, the key sites of the regulation of respiration are Complex IV (FCC = 0.74), ATP synthase (FCC = 0.61), and phosphate carrier (FCC = 0.60); these FCC(s) exceed considerably (~10-fold) those for normal oxidative tissues. In human BC cells, the outer mitochondrial membrane is characterized by an increased permeability towards adenine nucleotides, the mean value of the apparent K(m) for ADP being equal to 114.8 ± 13.6 μM. Our data support the two-compartment hypothesis of tumor metabolism, the high sum of FCC(s) showing structural and functional organization of mitochondrial respiratory chain and ATP synthasome as supercomplexes in human BC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.