ArticleRNA (New York, N.Y.)2012
Association of Argonaute proteins and microRNAs can occur after cell lysis.
Article in RNA (New York, N.Y.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
59 citing papers in PubMed, 85 citations in OpenAlex.
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- tatDB: a database of Ago1-mediated targets of transfer RNA fragments.Nucleic acids research · 2023Article
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- Integrative genome-wide analysis reveals EIF3A as a key downstream regulator of translational repressor protein Musashi 2 (MSI2).NAR cancer · 2022Article
- Next-generation sequencing: A new avenue to understand viral RNA-protein interactions.The Journal of biological chemistry · 2022Review
- Targeted RNA editing: novel tools to study post-transcriptional regulation.Molecular cell · 2022Review
- Approaches to Identify and Characterise the Post-Transcriptional Roles of lncRNAs in Cancer.Non-coding RNA · 2021Review
- Comparative Analysis of Transcriptome and sRNAs Expression Patterns in theInternational journal of molecular sciences · 2021Article
- miRNA Targets: From Prediction Tools to Experimental Validation.Methods and protocols · 2020Review
- Experimental Approaches to Identify Host Factors Important for Influenza Virus.Cold Spring Harbor perspectives in medicine · 2020Review
- An optimized chemical-genetic method for cell-specific metabolic labeling of RNA.Nature methods · 2020Article
- In-cell identification and measurement of RNA-protein interactions.Nature communications · 2019Article
- No evidence for viral small RNA production and antiviral function of Argonaute 2 in human cells.Scientific reports · 2019Article
- Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
MicroRNA (miRNA) target identification is a challenging but important endeavor. Global analyses of the direct mRNA targets of miRNAs have relied heavily upon immunopurification techniques, wherein a core protein component of the miRNA-protein complex, Argonaute (Ago), is immunoprecipitated to isolate associated RNAs. This approach involves the assumption that the selected RNAs were bound to the Ago protein in vivo and that the methodology did not significantly perturb endogenous interactions or produce novel interaction artifacts. To test whether RNAs that coimmunoprecipitate with human Ago were bound in vivo or could associate post-cell lysis, we used an experimental approach that distinguishes between these two origins of interaction. We show that a transfected miRNA mimic, but not a plasmid-expressed miRNA, can interact with human Ago proteins post-lysis. Our results have important implications for the design of miRNP immunoprecipitation experiments.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.