ArticleVector borne and zoonotic diseases (Larchmont, N.Y.)2012
Development of a multi-target TaqMan assay to detect eastern equine encephalitis virus variants in mosquitoes.
Article in Vector borne and zoonotic diseases (Larchmont, N.Y.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Inactivation of highly transmissible livestock and avian viruses including influenza A and Newcastle disease virus for molecular diagnostics.Frontiers in veterinary science · 2024Article
- Dynamics of eastern equine encephalitis virus during the 2019 outbreak in the Northeast United States.Current biology : CB · 2023Article
- Dynamics of Eastern equine encephalitis virus during the 2019 outbreak in the Northeast United States.medRxiv : the preprint server for health sciences · 2023Article
- Presence of mismatches between diagnostic PCR assays and coronavirus SARS-CoV-2 genome.Royal Society open science · 2020Article
- In silico re-assessment of a diagnostic RT-qPCR assay for universal detection of Influenza A viruses.Scientific reports · 2019Article
- Protective antibodies against Eastern equine encephalitis virus bind to epitopes in domains A and B of the E2 glycoprotein.Nature microbiology · 2019Article
- Evaluation of EZplex MTBC/NTM Real-Time PCR kit: diagnostic accuracy and efficacy in vaccination.Clinical and experimental vaccine research · 2018Article
- Optimized Lysis-Extraction Method Combined WithFrontiers in microbiology · 2018Article
- Analytical and clinical performance of a Chikungunya qRT-PCR for Central and South America.Diagnostic microbiology and infectious disease · 2017Article
- Evaluation of TaqMan qPCR System Integrating Two Identically Labelled Hydrolysis Probes in Single Assay.Scientific reports · 2017Article
- Article
- Insights into the recent emergence and expansion of eastern equine encephalitis virus in a new focus in the Northern New England USA.Parasites & vectors · 2015Article
- Vector-Host Interactions of Culiseta melanura in a Focus of Eastern Equine Encephalitis Virus Activity in Southeastern Virginia.PloS one · 2015Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Disease outbreaks caused by eastern equine encephalitis virus (EEEV; Togaviridae, Alphavirus) may be prevented by implementing effective surveillance and intervention strategies directed against the mosquito vector. Methods for EEEV detection in mosquitoes include a real-time reverse transcriptase PCR technique (TaqMan assay), but we report its failure to detect variants isolated in Connecticut in 2011, due to a single base-pair mismatch in the probe-binding site. To improve the molecular detection of EEEV, we developed a multi-target TaqMan assay by adding a second primer/probe set to provide redundant targets for EEEV detection. The multi-target TaqMan assay had similar performance characteristics to the conventional assay, but also detected newly-evolving strains of EEEV. The approach described here increases the reliability of the TaqMan assay by creating back-up targets for virus detection without sacrificing sensitivity or specificity.
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