ArticleEnvironmental science & technology2012
In vitro exposures in diesel exhaust atmospheres: resuspension of PM from filters versus direct deposition of PM from air.
Article in Environmental science & technology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed.
- Short-lived reactive components substantially contribute to particulate matter oxidative potential.Science advances · 2025Article
- Article
- Toxicity assessment of CeO₂ and CuO nanoparticles at the air-liquid interface using bioinspired condensational particle growth.Hygiene and environmental health advances · 2023Article
- Respiratory Health Effects of In Vivo Sub-Chronic Diesel and Biodiesel Exhaust Exposure.International journal of molecular sciences · 2023Article
- Article
- Comparison of biological responses between submerged, pseudo-air-liquid interface, and air-liquid interface exposure of A549 and differentiated THP-1 co-cultures to combustion-derived particles.Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering · 2022Article
- Co-culture of human alveolar epithelial (A549) and macrophage (THP-1) cells to study the potential toxicity of ambient PMToxicology research · 2020Article
- Mimicking the human respiratory system: Online in vitro cell exposure for toxicity assessment of welding fume aerosol.Journal of hazardous materials · 2020Article
- Particulate Matter Toxicity Is Nrf2 and Mitochondria Dependent: The Roles of Metals and Polycyclic Aromatic Hydrocarbons.Chemical research in toxicology · 2020Review
- New Approach Methods to Evaluate Health Risks of Air Pollutants: Critical Design Considerations for In Vitro Exposure Testing.International journal of environmental research and public health · 2020Review
- Application of a Quartz Crystal Microbalance to Measure the Mass Concentration of Combustion Particle Suspensions.Journal of aerosol science · 2019Article
- Understanding the Early Biological Effects of Isoprene-Derived Particulate Matter Enhanced by Anthropogenic Pollutants.Research report (Health Effects Institute) · 2019Article
- Cardiovascular effects of diesel exhaust inhalation: photochemically altered versus freshly emitted in mice.Journal of toxicology and environmental health. Part A · 2019Article
- Condensational particle growth device for reliable cell exposure at the air-liquid interface to nanoparticles.Aerosol science and technology : the journal of the American Association for Aerosol Research · 2019Article
- Evaluation of an Air Quality Health Index for Predicting the Mutagenicity of Simulated Atmospheres.Environmental science & technology · 2018Article
- Environmentally Persistent Free Radicals: Insights on a New Class of Pollutants.Environmental science & technology · 2018Article
- Gene Expression Profiling in Human Lung Cells Exposed to Isoprene-Derived Secondary Organic Aerosol.Environmental science & technology · 2017Article
- Regulating temperature and relative humidity in air-liquid interfaceToxicology research · 2017Article
- Diesel exhaust: current knowledge of adverse effects and underlying cellular mechanisms.Archives of toxicology · 2016Review
- Assessment of biological responses of EpiAirway 3-D cell constructs versus A549 cells for determining toxicity of ambient air pollution.Inhalation toxicology · 2016Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
One of the most widely used in vitro particulate matter (PM) exposures methods is the collection of PM on filters, followed by resuspension in a liquid medium, with subsequent addition onto a cell culture. To avoid disruption of equilibria between gases and PM, we have developed a direct in vitro sampling and exposure method (DSEM) capable of PM-only exposures. We hypothesize that the separation of phases and post-treatment of filter-collected PM significantly modifies the toxicity of the PM compared to direct deposition, resulting in a distorted view of the potential PM health effects. Controlled test environments were created in a chamber that combined diesel exhaust with an urban-like mixture. The complex mixture was analyzed using both the DSEM and concurrently collected filter samples. The DSEM showed that PM from test atmospheres produced significant inflammatory response, while the resuspension exposures at the same exposure concentration did not. Increasing the concentration of resuspended PM sixteen times was required to yield measurable IL-8 expression. Chemical analysis of the resuspended PM indicated a total absence of carbonyl compounds compared to the test atmosphere during the direct-exposures. Therefore, collection and resuspension of PM into liquid modifies its toxicity and likely leads to underestimating toxicity.
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Registered trials
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