ArticleMetabolomics : Official journal of the Metabolomic Society2012
Optimization of cold methanol quenching for quantitative metabolomics of Penicillium chrysogenum.
Article in Metabolomics : Official journal of the Metabolomic Society, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed.
- Biodesulfurization and activation of a thionated levofloxacin derivative: antimicrobial evaluation and LC-MS-based metabolic profiling.Naunyn-Schmiedeberg's archives of pharmacology · 2026Article
- Critical assessment of quenching and extraction/sample preparation methods for microorganisms in metabolomics.Metabolomics : Official journal of the Metabolomic Society · 2025Review
- 13 C-MFA helps to identify metabolic bottlenecks for improving malic acid production in Myceliophthora thermophila.Microbial cell factories · 2024Article
- Optimized Fast Filtration-Based Sampling and Extraction Enables Precise and Absolute Quantification of theMetabolites · 2023Article
- Influence of oxygen concentration on the metabolism ofEngineering in life sciences · 2023Article
- Enhanced production of γ-amino acid 3-amino-4-hydroxybenzoic acid by recombinant Corynebacterium glutamicum under oxygen limitation.Microbial cell factories · 2021Article
- Improved Sample Preparation for Untargeted Metabolomics Profiling of Escherichia coli.Microbiology spectrum · 2021Article
- Mapping of the benzoate metabolism by human gut microbiome indicates food-derived metagenome evolution.Scientific reports · 2021Article
- A Penicillium rubens platform strain for secondary metabolite production.Scientific reports · 2020Article
- GC-MS-Based Metabolomics for the Smut FungusFrontiers in molecular biosciences · 2020Article
- Time-resolved decoding of metabolic signatures of in vitro growth of the hemibiotrophic pathogen Colletotrichum sublineolum.Scientific reports · 2019Article
- Review of recent developments in GC-MS approaches to metabolomics-based research.Metabolomics : Official journal of the Metabolomic Society · 2018Review
- Quenching for Microalgal Metabolomics: A Case Study on the Unicellular Eukaryotic Green AlgaMetabolites · 2018Article
- Metabolome analysis-based design and engineering of a metabolic pathway in Corynebacterium glutamicum to match rates of simultaneous utilization of D-glucose and L-arabinose.Microbial cell factories · 2018Article
- Metabolomics Analysis of the Toxic Effects of the Production of Lycopene and Its Precursors.Frontiers in microbiology · 2018Article
- Analysis of Intracellular Metabolites from Microorganisms: Quenching and Extraction Protocols.Metabolites · 2017Review
- UHPLC-MS/MS determination of varietal thiol precursors in Sauvignon Blanc grapes.Scientific reports · 2017Article
- Quantitative metabolomics of the thermophilic methylotroph Bacillus methanolicus.Microbial cell factories · 2016Article
- Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A sampling procedure for quantitative metabolomics in Penicillium chrysogenum based on cold aqueous methanol quenching was re-evaluated and optimized to reduce metabolite leakage during sample treatment. The optimization study included amino acids and intermediates of the glycolysis and the TCA-cycle. Metabolite leakage was found to be minimal for a methanol content of the quenching solution (QS) of 40% (v/v) while keeping the temperature of the quenched sample near -20°C. The average metabolite recovery under these conditions was 95.7% (±1.1%). Several observations support the hypothesis that metabolite leakage from quenched mycelia of P. chrysogenum occurs by diffusion over the cell membrane. First, a prolonged contact time between mycelia and the QS lead to a somewhat higher extent of leakage. Second, when suboptimal quenching liquids were used, increased metabolite leakage was found to be correlated with lower molecular weight and with lower absolute net charge. The finding that lowering the methanol content of the quenching liquid reduces metabolite leakage in P. chrysogenum contrasts with recently published quenching studies for two other eukaryotic micro-organisms. This demonstrates that it is necessary to validate and, if needed, optimize the quenching conditions for each particular micro-organism. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11306-011-0367-3) contains supplementary material, which is available to authorized users.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.