ArticleNucleic acids research2012
The CR3 motif of Rrp44p is important for interaction with the core exosome and exosome function.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- How hydrolytic exoribonucleases impact human disease: Two sides of the same story.FEBS open bio · 2023Review
- Review
- Dissecting Trypanosoma brucei RRP44 function in the maturation of segmented ribosomal RNA using a regulated genetic complementation system.Nucleic acids research · 2023Article
- Trypanosoma brucei RRP44: a versatile enzyme for processing structured and non-structured RNA substrates.Nucleic acids research · 2023Article
- MPP6 stimulates both RRP6 and DIS3 to degrade a specified subset of MTR4-sensitive substrates in the human nucleus.Nucleic acids research · 2022Article
- Post-transcriptional control of cellular differentiation by the RNA exosome complex.Nucleic acids research · 2020Review
- The Bacterial Counterparts of the Eukaryotic Exosome: An Evolutionary Perspective.Methods in molecular biology (Clifton, N.J.) · 2020Review
- Trypanosoma brucei RRP44 is involved in an early stage of large ribosomal subunit RNA maturation.RNA biology · 2019Article
- Distinct and evolutionary conserved structural features of the human nuclear exosome complex.eLife · 2018Article
- DIS3 isoforms vary in their endoribonuclease activity and are differentially expressed within haematological cancers.The Biochemical journal · 2018Article
- The RNA Exosome Channeling and Direct Access Conformations Have Distinct In Vivo Functions.Cell reports · 2016Article
- Article
- The 3' to 5' Exoribonuclease DIS3: From Structure and Mechanisms to Biological Functions and Role in Human Disease.Biomolecules · 2015Review
- The ribonuclease DIS3 promotes let-7 miRNA maturation by degrading the pluripotency factor LIN28B mRNA.Nucleic acids research · 2015Article
- The 3'-5' exoribonuclease Dis3 regulates the expression of specific microRNAs in Drosophila wing imaginal discs.RNA biology · 2015Article
- The role of surgeons in building a personalized medicine program.Journal of surgical oncology · 2015Review
- Looking into the barrel of the RNA exosome.Nature structural & molecular biology · 2014Article
- Threading the barrel of the RNA exosome.Trends in biochemical sciences · 2013Review
- The exoribonuclease Dis3L2 defines a novel eukaryotic RNA degradation pathway.The EMBO journal · 2013Article
- Exonuclease hDIS3L2 specifies an exosome-independent 3'-5' degradation pathway of human cytoplasmic mRNA.The EMBO journal · 2013Article
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Authors and funding
5 authors.
Funding
Abstract
The 10-subunit RNA exosome is involved in a large number of diverse RNA processing and degradation events in eukaryotes. These reactions are carried out by the single catalytic subunit, Rrp44p/Dis3p, which is composed of three parts that are conserved throughout eukaryotes. The exosome is named for the 3' to 5' exoribonuclease activity provided by a large C-terminal region of the Rrp44p subunit that resembles other exoribonucleases. Rrp44p also contains an endoribonuclease domain. Finally, the very N-terminus of Rrp44p contains three Cys residues (CR3 motif) that are conserved in many eukaryotes but have no known function. These three conserved Cys residues cluster with a previously unrecognized conserved His residue in what resembles a metal-ion-binding site. Genetic and biochemical data show that this CR3 motif affects both endo- and exonuclease activity in vivo and both the nuclear and cytoplasmic exosome, as well as the ability of Rrp44p to associate with the other exosome subunits. These data provide the first direct evidence that the exosome-Rrp44p interaction is functionally important and also provides a molecular explanation for the functional defects when the conserved Cys residues are mutated.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.