ArticleCurrent microbiology2012
Transformation and conjugal transfer of foreign genes into the filamentous multicellular cyanobacteria (subsection V) Fischerella and Chlorogloeopsis.
Article in Current microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 47 citations in OpenAlex.
- Identification of a Far-Red Light-Inducible Promoter that Exhibits Light Intensity Dependency and Reversibility in a Cyanobacterium.ACS synthetic biology · 2023Article
- The Molecular Toolset and Techniques Required to Build Cyanobacterial Cell Factories.Advances in biochemical engineering/biotechnology · 2023Article
- Article
- Structural Determinants and Their Role in Cyanobacterial Morphogenesis.Life (Basel, Switzerland) · 2020Review
- The role of the cytoskeletal proteins MreB and FtsZ in multicellular cyanobacteria.FEBS open bio · 2020Article
- Function and Benefits of Natural Competence in Cyanobacteria: From Ecology to Targeted Manipulation.Life (Basel, Switzerland) · 2020Review
- Identification and characterization of novel filament-forming proteins in cyanobacteria.Scientific reports · 2020Article
- Evaluation and Comparison of the Efficiency of Transcription Terminators in Different Cyanobacterial Species.Frontiers in microbiology · 2020Article
- Emerging Species and Genome Editing Tools: Future Prospects in Cyanobacterial Synthetic Biology.Microorganisms · 2019Review
- CyanoGate: A Modular Cloning Suite for Engineering Cyanobacteria Based on the Plant MoClo Syntax.Plant physiology · 2019Article
- Genomics Approaches to Deciphering Natural Transformation in Cyanobacteria.Frontiers in microbiology · 2019Article
- Plasticity first: molecular signatures of a complex morphological trait in filamentous cyanobacteria.BMC evolutionary biology · 2017Article
- Role of PatS and cell type on the heterocyst spacing pattern in a filamentous branching cyanobacterium.FEMS microbiology letters · 2017Article
- Evolution of Chaperonin Gene Duplication in Stigonematalean Cyanobacteria (Subsection V).Genome biology and evolution · 2017Article
- Genomics of variation in nitrogen fixation activity in a population of the thermophilic cyanobacterium Mastigocladus laminosus.The ISME journal · 2017Article
- Premethylation of foreign DNA improves integrative transformation efficiency in Synechocystis sp. strain PCC 6803.Applied and environmental microbiology · 2015Article
- RfpA, RfpB, and RfpC are the Master Control Elements of Far-Red Light Photoacclimation (FaRLiP).Frontiers in microbiology · 2015Article
- Plasmid conjugation from proteobacteria as evidence for the origin of xenologous genes in cyanobacteria.Journal of bacteriology · 2014Article
- Broad-host-range vector system for synthetic biology and biotechnology in cyanobacteria.Nucleic acids research · 2014Article
- A world of cytochrome P450s.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Article
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5 authors at 1 institution in 1 country.
Funding
Abstract
Cyanobacteria of subsection V grow as filaments with asymmetrical cell divisions that can generate a true-branching phenotype. Members of the genera Fischerella and Chlorogloeopsis furthermore differentiate akinetes (spore-like resting stages), heterocysts (specialized in nitrogen fixation) and hormogonia (cell aggregates with gliding motility for colonization and dispersal). Genetic approaches to studying the complex morphology and differentiations of these prokaryotes require transformation techniques. For Fischerella and Chlorogloeopsis reliable protocols for introducing foreign genes are lacking. Here, we explored conjugation, electroporation, and biolistic DNA transfer methods in Fischerella and Chlorogloeopsis, using the cyanobacterial replicon pRL25C as a marker. We successfully transformed Fischerella muscicola PCC 7414 and Chlorogloeopsis fritschii PCC 6912 and were able to express the GFP reporter protein under two different promoters: the nitrogen regulated (p) glnA and the strong E. coli hybrid (p) trc. For Fischerella all methods worked, for Chlorogloeopsis electroporation was unsuccessful. For both strains conjugation delivered the most reproducible results, whereby partial removal of the exopolysaccharide sheath by salt washing was a critical step.
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