ArticleNephron. Physiology2012
An intact kidney slice model to investigate vasa recta properties and function in situ.
Article in Nephron. Physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 56 citations in OpenAlex.
- The kidney stroma in development and disease.Nature reviews. Nephrology · 2025Review
- Analysing the Renal Vasculature Using Super-Resolution Ultrasound Imaging: Considerations for Clinical and Research Applications.Diagnostics (Basel, Switzerland) · 2025Article
- Role of perivascular cells in kidney homeostasis, inflammation, repair and fibrosis.Nature reviews. Nephrology · 2023Review
- A novel functional role for the classic CNS neurotransmitters, GABA, glycine, and glutamate, in the kidney: potent and opposing regulators of the renal vasculature.American journal of physiology. Renal physiology · 2023Article
- Inflammatory mediators act at renal pericytes to elicit contraction of vasa recta and reduce pericyte density along the kidney medullary vascular network.Frontiers in physiology · 2023Article
- Nitric Oxide Signalling in Descending Vasa Recta after Hypoxia/Re-Oxygenation.International journal of molecular sciences · 2022Article
- Review
- Mediators of Regional Kidney Perfusion during Surgical Pneumo-Peritoneum Creation and the Risk of Acute Kidney Injury-A Review of Basic Physiology.Journal of clinical medicine · 2022Review
- Super-Resolution Ultrasound Imaging Can Quantify Alterations in Microbubble Velocities in the Renal Vasculature of Rats.Diagnostics (Basel, Switzerland) · 2022Article
- Calreticulin Shortage Results in Disturbance of Calcium Storage, Mitochondrial Disease, and Kidney Injury.Cells · 2022Article
- Pericyte-mediated constriction of renal capillaries evokes no-reflow and kidney injury following ischaemia.eLife · 2022Article
- Extracellular Nucleotides and P2 Receptors in Renal Function.Physiological reviews · 2020Review
- Anandamide and endocannabinoid system: an attractive therapeutic approach for cardiovascular disease.Therapeutic advances in cardiovascular disease · 2018Review
- Vasa recta pericyte density is negatively associated with vascular congestion in the renal medulla following ischemia reperfusion in rats.American journal of physiology. Renal physiology · 2017Article
- Pericytes, an overlooked player in vascular pathobiology.Pharmacology & therapeutics · 2017Review
- σ1-Receptor Agonism Protects against Renal Ischemia-Reperfusion Injury.Journal of the American Society of Nephrology : JASN · 2017Article
- TWEAK-Fn14 Signaling Activates Myofibroblasts to Drive Progression of Fibrotic Kidney Disease.Journal of the American Society of Nephrology : JASN · 2016Article
- Intravital and Kidney Slice Imaging of Podocyte Membrane Dynamics.Journal of the American Society of Nephrology : JASN · 2016Article
- High salt intake increases endothelin B receptor function in the renal medulla of rats.Life sciences · 2016Article
- High salt induces autocrine actions of ET-1 on inner medullary collecting duct NO production via upregulated ETB receptor expression.American journal of physiology. Regulatory, integrative and comparative physiology · 2016Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundMedullary blood flow is via vasa recta capillaries, which possess contractile pericytes. In vitro studies using isolated descending vasa recta show that pericytes can constrict/dilate descending vasa recta when vasoactive substances are present. We describe a live kidney slice model in which pericyte-mediated vasa recta constriction/dilation can be visualized in situ.
methodsConfocal microscopy was used to image calcein, propidium iodide and Hoechst labelling in 'live' kidney slices, to determine tubular and vascular cell viability and morphology. DIC video-imaging of live kidney slices was employed to investigate pericyte-mediated real-time changes in vasa recta diameter.
resultsPericytes were identified on vasa recta and their morphology and density were characterized in the medulla. Pericyte-mediated changes in vasa recta diameter (10-30%) were evoked in response to bath application of vasoactive agents (norepinephrine, endothelin-1, angiotensin-II and prostaglandin E(2)) or by manipulating endogenous vasoactive signalling pathways (using tyramine, L-NAME, a cyclo-oxygenase (COX-1) inhibitor indomethacin, and ATP release).
conclusionsThe live kidney slice model is a valid complementary technique for investigating vasa recta function in situ and the role of pericytes as regulators of vasa recta diameter. This technique may also be useful in exploring the role of tubulovascular crosstalk in regulation of medullary blood flow.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.