ArticleAmerican journal of physiology. Renal physiology2012
Inhibition of αENaC expression and ENaC activity following blockade of the circadian clock-regulatory kinases CK1δ/ε.
Article in American journal of physiology. Renal physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 46 citations in OpenAlex.
- Review
- Pharmacological targeting of casein kinase 1δ suppresses oncogenic NRAS-driven melanoma.Nature communications · 2024Article
- Mineralocorticoid Receptor and Sleep Quality in Chronic Kidney Disease.International journal of molecular sciences · 2024Review
- BMAL1 sex-specific effects in the neonatal mouse airway exposed to moderate hyperoxia.Physiological reports · 2024Article
- Knockout of the Circadian Clock Protein PER1 (Period1) Exacerbates Hypertension and Increases Kidney Injury in Dahl Salt-Sensitive Rats.Hypertension (Dallas, Tex. : 1979) · 2022Article
- Sleep Restriction and Recurrent Circadian Disruption Differentially Affects Blood Pressure, Sodium Retention, and Aldosterone Secretion.Frontiers in physiology · 2022Article
- Circadian Rhythm Effects on the Molecular Regulation of Physiological Systems.Comprehensive Physiology · 2021Review
- Environmental circadian disruption suppresses rhythms in kidney function and accelerates excretion of renal injury markers in urine of male hypertensive rats.American journal of physiology. Renal physiology · 2021Article
- Direct and indirect inhibition of the circadian clock protein Per1: effects on ENaC and blood pressure.American journal of physiology. Renal physiology · 2019Article
- Autonomic nerves and circadian control of renal function.Autonomic neuroscience : basic & clinical · 2019Review
- Chronobiology in nephrology: the influence of circadian rhythms on renal handling of drugs and renal disease treatment.International urology and nephrology · 2018Review
- Circadian clock-mediated regulation of blood pressure.Free radical biology & medicine · 2018Review
- Circadian regulation of renal function.Free radical biology & medicine · 2018Review
- Cystic fibrosis transmembrane conductance regulator-emerging regulator of cancer.Cellular and molecular life sciences : CMLS · 2018Review
- Altered Circadian Timing System-Mediated Non-Dipping Pattern of Blood Pressure and Associated Cardiovascular Disorders in Metabolic and Kidney Diseases.International journal of molecular sciences · 2018Review
- High dietary sodium causes dyssynchrony of the renal molecular clock in rats.American journal of physiology. Renal physiology · 2018Article
- Aldosterone-induced expression of ENaC-α is associated with activity of p65/p50 in renal epithelial cells.Journal of nephrology · 2017Article
- The Circadian Clock in the Regulation of Renal Rhythms.Journal of biological rhythms · 2015Review
- Transcriptional regulation of NHE3 and SGLT1 by the circadian clock protein Per1 in proximal tubule cells.American journal of physiology. Renal physiology · 2015Article
- Molecular and Cellular Mechanisms of Aldosterone Producing Adenoma Development.Frontiers in endocrinology · 2015Review
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7 authors at 2 institutions in 1 country.
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Abstract
Increasing evidence suggests that the circadian clock plays an important role in the control of renal function and blood pressure. We previously showed that the circadian clock protein Period (Per)1, positively regulates the expression of the rate limiting subunit of the renal epithelial sodium channel (αENaC), which contributes to blood pressure regulation. Casein kinases 1δ and 1ε (CK1δ/ε) are critical regulators of clock proteins. CK1δ/ε must phosphorylate the circadian clock protein Per1 in order for the latter to enter the nucleus. We used a commercially available CK1δ/ε inhibitor, PF670462, to test the effect of CK1δ/ε blockade and inhibited Per1 nuclear entry on αENaC in a model of the renal cortical collecting duct (mpkCCD(c14) cells). CK1δ/ε blockade prevented Per1 and Clock from interacting with an E-box from the αENaC promoter. CK1δ/ε inhibition reduced αENaC mRNA levels by <60%. A similar decrease in αENaC mRNA was observed following siRNA-mediated CK1δ/ε knock-down. Inhibition of CK1δ/ε effectively prevented the transcriptional response of αENaC to aldosterone, suggesting an interaction between the circadian clock and aldosterone-mediated regulation of αENaC. CK1δ/ε inhibition significantly reduced αENaC but increased Caveolin-1 membrane protein levels; transepithelial current, a measure of ENaC activity, was decreased. Importantly, single channel analysis in amphibian renal cells demonstrated a dramatic decrease in the number of patches with observable ENaC current following CK1δ/ε inhibition. The present study shows for the first time that CK1δ/ε inhibition and impaired Per1 nuclear entry results in decreased αENaC expression and ENaC activity, providing further support for direct control of ENaC by the circadian clock.
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