ArticleMicroscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada2012
High-resolution optical imaging of zebrafish larval ribbon synapse protein RIBEYE, RIM2, and CaV 1.4 by stimulation emission depletion microscopy.
Article in Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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Who cites it
25 citing papers in PubMed, 36 citations in OpenAlex.
- Electrophysiological Profiling of Exocytosis during Early-Stage Development of the Zebrafish Lateral Line.eNeuro · 2026Article
- Article
- Electrophysiological profiling of exocytosis during early-stage development of the zebrafish lateral line.bioRxiv : the preprint server for biology · 2025Article
- Role of Ribeye PXDLS/T-binding cleft in normal synaptic ribbon function.bioRxiv : the preprint server for biology · 2023Article
- Super-Resolution Imaging Reveals the Nanoscale Distributions of Dystroglycan and Integrin Itga7 in Zebrafish Muscle Fibers.Biomedicines · 2023Article
- Imaging Approaches to Investigate Pathophysiological Mechanisms of Brain Disease in Zebrafish.International journal of molecular sciences · 2023Review
- Super-resolution STED imaging in the inner and outer whole-mount mouse retina.Frontiers in ophthalmology · 2023Article
- Synaptotagmins 1 and 7 in vesicle release from rods of mouse retina.Experimental eye research · 2022Article
- Adhesion GPCR Latrophilin 3 regulates synaptic function of cone photoreceptors in a trans-synaptic manner.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Biochemistry and physiology of zebrafish photoreceptors.Pflugers Archiv : European journal of physiology · 2021Review
- Transmission at rod and cone ribbon synapses in the retina.Pflugers Archiv : European journal of physiology · 2021Review
- Resting and stimulated mouse rod photoreceptors show distinct patterns of vesicle release at ribbon synapses.The Journal of general physiology · 2020Article
- Molecular Assembly and Structural Plasticity of Sensory Ribbon Synapses-A Presynaptic Perspective.International journal of molecular sciences · 2020Review
- Simultaneous Release of Multiple Vesicles from Rods Involves Synaptic Ribbons and Syntaxin 3B.Biophysical journal · 2020Article
- A Picture Worth a Thousand Molecules-Integrative Technologies for Mapping Subcellular Molecular Organization and Plasticity in Developing Circuits.Frontiers in synaptic neuroscience · 2020Article
- Voltage- and calcium-gated ion channels of neurons in the vertebrate retina.Progress in retinal and eye research · 2019Review
- Voltage-Gated Calcium Channels: Key Players in Sensory Coding in the Retina and the Inner Ear.Physiological reviews · 2018Review
- Endocytosis sustains release at photoreceptor ribbon synapses by restoring fusion competence.The Journal of general physiology · 2018Article
- Leveraging Zebrafish to Study Retinal Degenerations.Frontiers in cell and developmental biology · 2018Review
- Expansion microscopy of zebrafish for neuroscience and developmental biology studies.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The synaptic ribbon is a unique presynaptic structure with an intricate morphology in photoreceptors. Because of the resolution limit in conventional fluorescence microscopy, investigating ribbon protein locations has been challenging, especially in the early development stages of model animals. Here, we used stimulated emission depletion microscopy, a super-resolution imaging technique, to look at retina sections in 4 days post-fertilization (dpf) zebrafish. We observed that in photoreceptor cells, RIBEYE and RIM2 are expressed along the synaptic ribbon, with RIM2 consistently located inside of the horseshoe-shaped synaptic ribbon structure with RIBEYE located on the outside. The L-type calcium channel subunit, CACNA1F, exhibited small spot-like staining beneath the RIM2 and RIBEYE structures. Using morpholino antisense oligonucleotides to knock down RIBEYE expression, we observed fewer and shorter ribbons in the photoreceptor outer plexiform layers of 4 dpf fish retina as well as a reduction in RIM2 expression. The clustering of CACNA1F in these blind fish was no longer observed, but instead showed a diffuse expression in the photoreceptor terminal.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.