ArticleNeurogastroenterology and motility2012
Impaired propulsive motility in the distal but not proximal colon of BK channel β1-subunit knockout mice.
Article in Neurogastroenterology and motility, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- Rothia mucilaginosa alleviates constipation through gut microbiota remodeling and neuroactive pathway activation.BMC microbiology · 2026Article
- DRG afferents that mediate physiologic and pathologic mechanosensation from the distal colon.Cell · 2023Article
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- Inhibitory Effects of Dexmedetomidine and Propofol on Gastrointestinal Tract Motility Involving Impaired Enteric Glia CaNeurochemical research · 2021Article
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- Pyridostigmine bromide exposure creates chronic, underlying neuroimmune disruption in the gastrointestinal tract and brain that alters responses to palmitoylethanolamide in a mouse model of Gulf War Illness.Neuropharmacology · 2020Article
- Recommendations for evaluation of bladder and bowel function in pre-clinical spinal cord injury research.The journal of spinal cord medicine · 2020Review
- Gastrointestinal neuroimmune disruption in a mouse model of Gulf War illness.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- NF-κB/c-Rel deficiency causes Parkinson's disease-like prodromal symptoms and progressive pathology in mice.Translational neurodegeneration · 2019Article
- Upregulation of L-type calcium channels in colonic inhibitory motoneurons of P/Q-type calcium channel-deficient mice.American journal of physiology. Gastrointestinal and liver physiology · 2016Article
- High-fat diet-induced obesity alters nitric oxide-mediated neuromuscular transmission and smooth muscle excitability in the mouse distal colon.American journal of physiology. Gastrointestinal and liver physiology · 2016Article
- Role of BKBioMed research international · 2016Article
- Western blot analysis of BK channel β1-subunit expression should be interpreted cautiously when using commercially available antibodies.Physiological reports · 2014Article
- Ca2+ responses in enteric glia are mediated by connexin-43 hemichannels and modulate colonic transit in mice.Gastroenterology · 2014Article
- (2R,3S,2'' R,3''R)-manniflavanone, a new gastrointestinal smooth muscle L-type calcium channel inhibitor, which underlies the spasmolytic properties of Garcinia buchananii stem bark extract.Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
backgroundLarge-conductance Ca(2+) -activated K(+) (BK) channels regulate smooth muscle tone. The BK channel β1-subunit increases Ca(2+) sensitivity of the α-subunit in smooth muscle. We studied β1-subunit knockout (KO) mice to determine if gastrointestinal (GI) motility was altered.
methodsColonic and intestinal longitudinal muscle reactivity to bethanechol and colonic migrating motor complexes (CMMCs) were measured in vitro. Gastric emptying and small intestinal transit were measured in vivo. Colonic motility was assessed in vivo by measuring fecal output and glass bead expulsion time. Myoelectric activity of distal colon smooth muscle was measured in vitro using intracellular microelectrodes. KEY
resultsBethanechol-induced contractions were larger in the distal colon of β1-subunit KO compared to wild type (WT) mice; there were no differences in bethanechol reactivity in the duodenum, ileum, or proximal colon of WT vsβ1-subunit KO mice. There were more retrogradely propagated CMMCs in the distal colon of β1-subunit KO compared to WT mice. Gastrointestinal transit was unaffected by β1-subunit KO. Fecal output was decreased and glass bead expulsion times were increased in β1-subunit KO mice. Membrane potential of distal colon smooth muscle cells from β1-subunit KO mice was depolarized with higher action potential frequency compared to WT mice. Paxilline (BK channel blocker) depolarized smooth muscle cells and increased action potential frequency in WT distal colon. CONCLUSIONS & INFERENCES: BK channels play a prominent role in smooth muscle function only in the distal colon of mice. Defects in smooth muscle BK channel function disrupt colonic motility causing constipation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.