ArticleEnzyme research2012
Use of fluorochrome-labeled inhibitors of caspases to detect neuronal apoptosis in the whole-mounted lamprey brain after spinal cord injury.
Article in Enzyme research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 40 citations in OpenAlex.
- Caspase-8 expression in CD8Science advances · 2025Article
- Prenatal alcohol exposure promotes nerve injury-induced pathological pain following morphine treatment via NLRP3-mediated peripheral and central proinflammatory immune actions.Brain, behavior, and immunity · 2025Article
- Combined RhoA morpholino and ChABC treatment protects identified lamprey neurons from retrograde apoptosis after spinal cord injury.Frontiers in cellular neuroscience · 2023Article
- Chondroitinase ABC Promotes Axon Regeneration and Reduces Retrograde Apoptosis Signaling in Lamprey.Frontiers in cell and developmental biology · 2021Article
- Source of Early Regenerating Axons in Lamprey Spinal Cord Revealed by Wholemount Optical Clearing with BABB.Cells · 2020Article
- PTPσ Knockdown in Lampreys Impairs Reticulospinal Axon Regeneration and Neuronal Survival After Spinal Cord Injury.Frontiers in cellular neuroscience · 2020Article
- Inhibition of Gamma-Secretase Promotes Axon Regeneration After a Complete Spinal Cord Injury.Frontiers in cell and developmental biology · 2020Article
- Role of axon resealing in retrograde neuronal death and regeneration after spinal cord injury.Neural regeneration research · 2019Review
- Regenerative capacity in the lamprey spinal cord is not altered after a repeated transection.PloS one · 2019Article
- Data on the effect of a muscimol treatment in caspase activation in descending neurons of lampreys after a complete spinal cord injury.Data in brief · 2018Article
- RhoA activation in axotomy-induced neuronal death.Experimental neurology · 2018Article
- GABA promotes survival and axonal regeneration in identifiable descending neurons after spinal cord injury in larval lampreys.Cell death & disease · 2018Article
- Role of Caspase-8 and Fas in Cell Death After Spinal Cord Injury.Frontiers in molecular neuroscience · 2018Review
- Retrograde Activation of the Extrinsic Apoptotic Pathway in Spinal-Projecting Neurons after a Complete Spinal Cord Injury in Lampreys.BioMed research international · 2017Article
- Spinal motor neurons are regenerated after mechanical lesion and genetic ablation in larval zebrafish.Development (Cambridge, England) · 2016Article
- Reducing synuclein accumulation improves neuronal survival after spinal cord injury.Experimental neurology · 2016Article
- Expression of RBMX in the light-induced damage of rat retina in vivo.Cellular and molecular neurobiology · 2015Article
- "Bad regenerators" die after spinal cord injury: insights from lampreys.Neural regeneration research · 2015Article
- Spatiotemporal pattern of TRAF3 expression after rat spinal cord injury.Journal of molecular histology · 2014Article
- Selective expression of CSPG receptors PTPσ and LAR in poorly regenerating reticulospinal neurons of lamprey.The Journal of comparative neurology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Apoptosis is a major feature in neural development and important in traumatic diseases. The presence of active caspases is a widely accepted marker of apoptosis. We report here the development of a method to study neuronal apoptotic death in whole-mounted brain preparations using fluorochrome-labeled inhibitors of caspases (FLICA). As a model we used axotomy-induced retrograde neuronal death in the CNS of larval sea lampreys. Once inside the cell, the FLICA reagents bind covalently to active caspases causing apoptotic cells to fluoresce, whereas nonapoptotic cells remain unstained. The fluorescent probe, the poly caspase inhibitor FAM-VAD-FMK, was applied to whole-mounted brain preparations of larval sea lampreys 2 weeks after a complete spinal cord (SC) transection. Specific labeling occurred only in identifiable spinal-projecting neurons of the brainstem previously shown to undergo apoptotic neuronal death at later times after SC transection. These neurons also exhibited intense labeling 2 weeks after a complete SC transection when a specific caspase-8 inhibitor (FAM-LETD-FMK) served as the probe. In this study we show that FLICA reagents can be used to detect specific activated caspases in identified neurons of the whole-mounted lamprey brain. Our results suggest that axotomy may cause neuronal apoptosis by activation of the extrinsic apoptotic pathway.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.