ArticleOxidative medicine and cellular longevity2012
Detailed analysis of apoptosis and delayed luminescence of human leukemia Jurkat T cells after proton irradiation and treatments with oxidant agents and flavonoids.
Article in Oxidative medicine and cellular longevity, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 29 citations in OpenAlex.
- Article
- Effect of Astaxanthin on Tissue Transglutaminase and Cytoskeletal Protein Expression in Amyloid-Beta Stressed Olfactory Ensheathing Cells: Molecular and Delayed Luminescence Studies.Antioxidants (Basel, Switzerland) · 2023Article
- Relationship between delayed luminescence emission and mitochondrial status in Saccharomyces cerevisiae.Scientific reports · 2022Article
- Exploring the behaviour of water in glycerol solutions by using delayed luminescence.PloS one · 2018Article
- Simple discrimination of sub-cycling cells by propidium iodide flow cytometric assay in Jurkat cell samples with extensive DNA fragmentation.Cell cycle (Georgetown, Tex.) · 2018Article
- Reprogramming of leukemic cell metabolism through the naphthoquinonic compound Quambalarine B.Oncotarget · 2017Article
- Pulse frequency dependency of photobiomodulation on the bioenergetic functions of human dental pulp stem cells.Scientific reports · 2017Article
- Established Human Cell Lines as Models to Study Anti-leukemic Effects of Flavonoids.Current genomics · 2017Article
- Proton Beams Inhibit Proliferation of Breast Cancer Cells by Altering DNA Methylation Status.Journal of Cancer · 2016Article
- Molecular mechanisms of leptin and pro-apoptotic signals induced by menadione in HepG2 cells.Saudi journal of biological sciences · 2014Article
- Quercetin as a fluorescent probe for the ryanodine receptor activity in Jurkat cells.Pflugers Archiv : European journal of physiology · 2013Article
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Authors and funding
15 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Following previous work, we investigated in more detail the relationship between apoptosis and delayed luminescence (DL) in human leukemia Jurkat T cells under a wide variety of treatments. We used menadione and hydrogen peroxide to induce oxidative stress and two flavonoids, quercetin, and epigallocatechin gallate, applied alone or in combination with menadione or H(2)O(2). 62 MeV proton beams were used to irradiate cells under a uniform dose of 2 or 10 Gy, respectively. We assessed apoptosis, cell cycle distributions, and DL. Menadione, H(2)O(2) and quercetin were potent inducers of apoptosis and DL inhibitors. Quercetin decreased clonogenic survival and the NAD(P)H level in a dose-dependent manner. Proton irradiation with 2 Gy but not 10 Gy increased the apoptotic rate. However, both doses induced a substantial G(2)/M arrest. Quercetin reduced apoptosis and prolonged the G(2)/M arrest induced by radiation. DL spectroscopy indicated that proton irradiation disrupted the electron flow within Complex I of the mitochondrial respiratory chain, thus explaining the massive necrosis induced by 10 Gy of protons and also suggested an equivalent action of menadione and quercetin at the level of the Fe/S center N2, which may be mediated by their binding to a common site within Complex I, probably the rotenone-binding site.
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