ArticlePloS one2012
Design and characterization of auxotrophy-based amino acid biosensors.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 47 citations in OpenAlex.
- Systematic design of auxotrophic strains and media conditions to probe metabolic functions in E. coli.PLoS computational biology · 2026Article
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- Asymmetric metabolic adaptations undermine stability in microbial syntrophy.ISME communications · 2025Article
- A versatile microbial platform as a tunable whole-cell chemical sensor.Nature communications · 2024Article
- Genome-Wide Pathway Exploration of theMicroorganisms · 2023Article
- Recent advances in screening amino acid overproducers.Engineering microbiology · 2023Review
- Rational engineering of a synthetic insect-bacterial mutualism.Current biology : CB · 2022Article
- Proline codon pair selection determines ribosome pausing strength and translation efficiency in bacteria.Communications biology · 2021Article
- UvrY is required for the full virulence ofVirulence · 2020Article
- Enhancement of amino acid production and secretion byMetabolic engineering communications · 2020Article
- Development ofFrontiers in microbiology · 2020Article
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- Bioassay for Determining the Concentrations of Caffeine and Individual Methylxanthines in Complex Samples.Applied and environmental microbiology · 2019Article
- Erwinia amylovora Auxotrophic Mutant Exometabolomics and Virulence on Apples.Applied and environmental microbiology · 2019Article
- Interkingdom microbial consortia mechanisms to guide biotechnological applications.Microbial biotechnology · 2018Review
- Overexpression of serine acetyltransferase in maize leaves increases seed-specific methionine-rich zeins.Plant biotechnology journal · 2018Article
- Whole-cell Escherichia coli lactate biosensor for monitoring mammalian cell cultures during biopharmaceutical production.Biotechnology and bioengineering · 2017Article
- Experimental Evolution of Metabolic Dependency in Bacteria.PLoS genetics · 2016Article
- Privatization of cooperative benefits stabilizes mutualistic cross-feeding interactions in spatially structured environments.The ISME journal · 2016Article
- Synthetic biology to access and expand nature's chemical diversity.Nature reviews. Microbiology · 2016Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Efficient and inexpensive methods are required for the high-throughput quantification of amino acids in physiological fluids or microbial cell cultures. Here we develop an array of Escherichia coli biosensors to sensitively quantify eleven different amino acids. By using online databases, genes involved in amino acid biosynthesis were identified that - upon deletion - should render the corresponding mutant auxotrophic for one particular amino acid. This rational design strategy suggested genes involved in the biosynthesis of arginine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, threonine, tryptophan, and tyrosine as potential genetic targets. A detailed phenotypic characterization of the corresponding single-gene deletion mutants indeed confirmed that these strains could neither grow on a minimal medium lacking amino acids nor transform any other proteinogenic amino acid into the focal one. Site-specific integration of the egfp gene into the chromosome of each biosensor decreased the detection limit of the GFP-labeled cells by 30% relative to turbidometric measurements. Finally, using the biosensors to determine the amino acid concentration in the supernatants of two amino acid overproducing E. coli strains (i.e. ΔhisL and ΔtdcC) both turbidometrically and via GFP fluorescence emission and comparing the results to conventional HPLC measurements confirmed the utility of the developed biosensor system. Taken together, our study provides not only a genotypically and phenotypically well-characterized set of publicly available amino acid biosensors, but also demonstrates the feasibility of the rational design strategy used.
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