ArticlePloS one2012
Identification and characterization of Eimeria tenella apical membrane antigen-1 (AMA1).
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Molecular characterization and protective efficacy of silent information regulator 2A from Eimeria tenella.Parasites & vectors · 2016Trial
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- Immunoinformatics-Aided Design of a Multiepitope Vaccine AgainstBioinformatics and biology insights · 2025Article
- Eimeria: Navigating complex intestinal ecosystems.PLoS pathogens · 2024Review
- Efficacy of dietary supplements ofOpen veterinary journal · 2024Article
- The microneme adhesive repeat domain of MIC3 protein determined the site specificity ofFrontiers in immunology · 2023Article
- Preliminary evaluation of the protective effects of recombinant AMA1 and IMP1 against Eimeria stiedae infection in rabbits.Parasites & vectors · 2022Article
- Molecular characterization of glyceraldehyde-3-phosphate dehydrogenase from Eimeria tenella.Parasitology research · 2022Article
- Eimeria proteins: order amidst disorder.Parasites & vectors · 2022Review
- Combined oral immunization with probiotics Entercoccus faecalis delivering surface-anchoredFrontiers in immunology · 2022Article
- Anticoccidial activity of natural plants extracts mixture againstFrontiers in veterinary science · 2022Article
- Curcumin reduces enteric isoprostane 8-iso-PGF2α and prostaglandin GF2α in specific pathogen-free Leghorn chickens challenged with Eimeria maxima.Scientific reports · 2021Article
- Molecular characterization and functional analysis of Eimeria tenella citrate synthase.Parasitology research · 2021Article
- Analysis of Genetic Diversity in Indian Isolates ofVaccines · 2021Article
- Eimeria tenella Eimeria-specific protein that interacts with apical membrane antigen 1 (EtAMA1) is involved in host cell invasion.Parasites & vectors · 2020Article
- Protective effects of a food-grade recombinant Lactobacillus plantarum with surface displayed AMA1 and EtMIC2 proteins of Eimeria tenella in broiler chickens.Microbial cell factories · 2020Article
- Article
- Further investigation of the characteristics and biological function of Eimeria tenella apical membrane antigen 1.Parasite (Paris, France) · 2020Article
- Effects of host fatty acid-binding protein 4 on Eimeria tenella sporozoites invasion of cells.Parasitology research · 2019Article
- The effect of autophagy on the survival and invasive activity of Eimeria tenella sporozoites.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Apical membrane antigen-1 (AMA1) is a micronemal protein of apicomplexan parasites that appears to be essential during the invasion of host cells. In this study, a full-length cDNA of AMA1 was identified from Eimeria tenella (Et) using expressed sequence tag and the rapid amplification of cDNA ends technique. EtAMA1 had an open reading frame of 1608 bp encoding a protein of 535 amino acids. Quantitative real-time PCR analysis revealed that EtAMA1 was expressed at higher levels in sporozoites than in the other developmental stages (unsporulated oocysts, sporulated oocysts and second-generation merozoites). The ectodomain sequence was expressed as recombinant EtAMA1 (rEtAMA1) and rabbit polyclonal antibodies raised against the rEtAMA1 recognized a 58-kDa native parasite protein by Western Blotting and had a potent inhibitory effect on parasite invasion, decreasing it by approximately 70%. Immunofluorescence analysis and immunohistochemistry analysis showed EtAMA1 might play an important role in sporozoite invasion and development.
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