ArticlePloS one2012
Perivascular-like cells contribute to the stability of the vascular network of osteogenic tissue formed from cell sheet-based constructs.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 52 citations in OpenAlex.
- The multifaceted roles of MCAM in development, homeostasis, pathological conditions, and cancer.Journal of molecular medicine (Berlin, Germany) · 2025Review
- Bone Spheroid Development Under Flow Conditions with Mesenchymal Stem Cells and Human Umbilical Vein Endothelial Cells in a 3D Porous Hydrogel Supplemented with Hydroxyapatite.Gels (Basel, Switzerland) · 2024Article
- Application of mesenchymal stem cell sheet for regeneration of craniomaxillofacial bone defects.Stem cell research & therapy · 2023Review
- Growth Factor-Free Vascularization of Marine-Origin Collagen Sponges Using Cryopreserved Stromal Vascular Fractions from Human Adipose Tissue.Marine drugs · 2022Article
- Bioinks Enriched with ECM Components Obtained by Supercritical Extraction.Biomolecules · 2022Article
- Preparation and characterization of human keratinocyte-fibroblast cell sheets constructed using PNIAM-co-AM grafted surfaces for burn wound healing.Journal of materials science. Materials in medicine · 2020Article
- Vascularized Microfluidics and the Blood-Endothelium Interface.Micromachines · 2019Review
- An overview on small molecule-induced differentiation of mesenchymal stem cells into beta cells for diabetic therapy.Stem cell research & therapy · 2019Review
- Recent advances in cell sheet technology for bone and cartilage regeneration: from preparation to application.International journal of oral science · 2019Review
- Microfluidic-Based 3D Engineered Microvascular Networks and Their Applications in Vascularized Microtumor Models.Micromachines · 2018Review
- Concise Review: The Regenerative Journey of Pericytes Toward Clinical Translation.Stem cells (Dayton, Ohio) · 2018Review
- [Application of cell sheet technology in bone and cartilage tissue engineering].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2018Review
- Perivascular cells and tissue engineering: Current applications and untapped potential.Pharmacology & therapeutics · 2017Review
- Pre-vascularization Enhances Therapeutic Effects of Human Mesenchymal Stem Cell Sheets in Full Thickness Skin Wound Repair.Theranostics · 2017Article
- The application of cell sheet engineering in the vascularization of tissue regeneration.Regenerative medicine · 2016Review
- Effect of prevascularization on in vivo vascularization of poly(propylene fumarate)/fibrin scaffolds.Biomaterials · 2016Article
- Pericyte-targeting drug delivery and tissue engineering.International journal of nanomedicine · 2016Review
- A multilayered microfluidic blood vessel-like structure.Biomedical microdevices · 2015Article
- Engineering clinically relevant volumes of vascularized bone.Journal of cellular and molecular medicine · 2015Review
- Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In recent years several studies have been supporting the existence of a close relationship in terms of function and progeny between Mesenchymal Stem Cells (MSCs) and Pericytes. This concept has opened new perspectives for the application of MSCs in Tissue Engineering (TE), with special interest for the pre-vascularization of cell dense constructs. In this work, cell sheet technology was used to create a scaffold-free construct composed of osteogenic, endothelial and perivascular-like (CD146(+)) cells for improved in vivo vessel formation, maturation and stability. The CD146 pericyte-associated phenotype was induced from human bone marrow mesenchymal stem cells (hBMSCs) by the supplementation of standard culture medium with TGF-β1. Co-cultured cell sheets were obtained by culturing perivascular-like (CD146(+)) cells and human umbilical vein endothelial cells (HUVECs) on an hBMSCs monolayer maintained in osteogenic medium for 7 days. The perivascular-like (CD146(+)) cells and the HUVECs migrated and organized over the collagen-rich osteogenic cell sheet, suggesting the existence of cross-talk involving the co-cultured cell types. Furthermore the presence of that particular ECM produced by the osteoblastic cells was shown to be the key regulator for the singular observed organization. The osteogenic and angiogenic character of the proposed constructs was assessed in vivo. Immunohistochemistry analysis of the explants revealed the integration of HUVECs with the host vasculature as well as the osteogenic potential of the created construct, by the expression of osteocalcin. Additionally, the analysis of the diameter of human CD146 positive blood vessels showed a higher mean vessel diameter for the co-cultured cell sheet condition, reinforcing the advantage of the proposed model regarding blood vessels maturation and stability and for the in vitro pre-vascularization of TE constructs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.