ArticleDiabetologia2012
Modulation of gut microbiota by antibiotics improves insulin signalling in high-fat fed mice.
Article in Diabetologia, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 147 papers.
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Who cites it
147 citing papers in PubMed, 308 citations in OpenAlex.
- Changes in Gut Microbiome after Bariatric Surgery Versus Medical Weight Loss in a Pilot Randomized Trial.Obesity surgery · 2019Trial
- Short-Term Microbiota Manipulation and Forearm Substrate Metabolism in Obese Men: A Randomized, Double-Blind, Placebo-Controlled Trial.Obesity facts · 2018Trial
- Type 1 diabetic patients with peripheral neuropathy have pan-enteric prolongation of gastrointestinal transit times and an altered caecal pH profile.Diabetologia · 2017Trial
- Trial
- Early postnatal antibiotic-associated gut microbiota alterations might promote long-term lipid metabolism via brown adipose tissue metabolic programming.Gut microbes · 2026Article
- One Anastomosis Gastric Bypass Versus Single Anastomosis Sleeve Jejunal Bypass: Does Duodenal Exclusion Matter?Obesity surgery · 2026Review
- Prebiotic Diet Enhances Suppression of Tumor Necrosis Factor-Alpha Production in Response to Lipopolysaccharide after Exhaustive Exercise.Neuroimmunomodulation · 2026Article
- Neuroprotective effects ofFrontiers in nutrition · 2026Article
- The hidden cost of antibiotics: microbial dysbiosis-induced SCFA imbalance and its underlying mechanisms.Frontiers in immunology · 2026Review
- The mechanisms underlying COVID-19 induced insulin resistance: a narrative review.Frontiers in endocrinology · 2026Review
- Gut microbiota and associated metabolites: key players in high-fat diet-induced chronic diseases.Gut microbes · 2025Review
- Modulating the Gut-Muscle Axis: Increasing SCFA-Producing Gut Microbiota Commensals and Decreasing Endotoxin Production to Mitigate Cancer Cachexia.Microorganisms · 2025Review
- The Microbiota and Evolution of Obesity.Endocrine reviews · 2025Review
- Targeting the TLR4 axis with microbiota-oriented interventions and innovations in diabetes therapy: a narrative review.Frontiers in immunology · 2025Review
- Cancer Cachexia.Advances in experimental medicine and biology · 2025Review
- Gut microbiota is involved in the exacerbation of adrenal glucocorticoid steroidogenesis in diabetic animals by activation of the TLR4 pathway.Frontiers in endocrinology · 2025Article
- Gut microbiota dynamics in KK-Ay mice: restoration following antibiotic treatment.Folia microbiologica · 2024Article
- Gut bacteria are essential for development of an invasive bark beetle by regulating glucose transport.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- The individual response to antibiotics and diet - insights into gut microbial resilience and host metabolism.Nature reviews. Endocrinology · 2024Review
- Ginsenoside RgNutrients · 2024Article
87 more citing papers are in PubMed but not listed here.
Corrections and comments
- Expression of concern · 2017-05-15Concerns/Issues about Data · Concerns/Issues about Image · Duplication of/in Image ·
- Expression of concern
Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aims/hypothesisA high-fat dietary intake induces obesity and subclinical inflammation, which play important roles in insulin resistance. Recent studies have suggested that increased concentrations of circulating lipopolysaccharide (LPS), promoted by changes in intestinal permeability, may have a pivotal role in insulin resistance. Thus, we investigated the effect of gut microbiota modulation on insulin resistance and macrophage infiltration.
methodsSwiss mice were submitted to a high-fat diet with antibiotics or pair-feeding for 8 weeks. Metagenome analyses were performed on DNA samples from mouse faeces. Blood was collected to determine levels of glucose, insulin, LPS, cytokines and acetate. Liver, muscle and adipose tissue proteins were analysed by western blotting. In addition, liver and adipose tissue were analysed, blinded, using histology and immunohistochemistry.
resultsAntibiotic treatment greatly modified the gut microbiota, reducing levels of Bacteroidetes and Firmicutes, overall bacterial count and circulating LPS levels. This modulation reduced levels of fasting glucose, insulin, TNF-α and IL-6; reduced activation of toll-like receptor 4 (TLR4), c-Jun N-terminal kinase (JNK), inhibitor of κ light polypeptide gene enhancer in B cells, kinase β (IKKβ) and phosphorylated IRS-1 Ser307; and consequently improved glucose tolerance and insulin tolerance and action in metabolically active tissues. In addition, there was an increase in portal levels of circulating acetate, which probably contributed to an increase in 5'-AMP-activated protein kinase (AMPK) phosphorylation in mice. We observed a striking reduction in crown-like structures (CLS) and F4/80(+) macrophage cells in the adipose tissue of antibiotic-treated mice. CONCLUSIONS/
interpretationThese results suggest that modulation of gut microbiota in obesity can improve insulin signalling and glucose tolerance by reducing circulating LPS levels and inflammatory signalling. Modulation also appears to increase levels of circulating acetate, which activates AMPK and finally leads to reduced macrophage infiltration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.