ArticleBiophysical journal2012
Dynamic force spectroscopy on supported lipid bilayers: effect of temperature and sample preparation.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- AFM to Study Pore-Forming Proteins.Methods in molecular biology (Clifton, N.J.) · 2027Article
- The intricate link between membrane lipid structure and composition and membrane structural properties in bacterial membranes.Chemical science · 2024Review
- Temperature-Induced Restructuring of Mycolic Acid Bilayers Modeling theMolecules (Basel, Switzerland) · 2024Article
- Article
- Malaria parasites release vesicle subpopulations with signatures of different destinations.EMBO reports · 2022Article
- Membrane elasticity modulated by cholesterol in model of porcine eye lens-lipid membrane.Experimental eye research · 2022Article
- Erythrocyte Membrane Nanomechanical Rigidity Is Decreased in Obese Patients.International journal of molecular sciences · 2022Article
- Lipid Self-Assemblies under the Atomic Force Microscope.International journal of molecular sciences · 2021Review
- Mechanical properties of the high cholesterol-containing membrane: An AFM study.Biochimica et biophysica acta. Biomembranes · 2021Article
- Modulation of lipid membrane structural and mechanical properties by a peptidomimetic derived from reduced amide scaffold.Biochimica et biophysica acta. Biomembranes · 2017Article
- Review
- Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers.Journal of visualized experiments : JoVE · 2015Article
Corrections and comments
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Authors and funding
4 authors.
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Abstract
Biological membranes are constantly exposed to forces. The stress-strain relation in membranes determines the behavior of many integral membrane proteins or other membrane related-proteins that show a mechanosensitive behavior. Here, we studied by force spectroscopy the behavior of supported lipid bilayers (SLBs) subjected to forces perpendicular to their plane. We measured the lipid bilayer mechanical properties and the force required for the punch-through event characteristic of atomic force spectroscopy on SLBs as a function of the interleaflet coupling. We found that for an uncoupled bilayer, the overall tip penetration occurs sequentially through the two leaflets, giving rise to two penetration events. In the case of a bilayer with coupled leaflets, penetration of the atomic force microscope tip always occurred in a single step. Considering the dependence of the jump-through force value on the tip speed, we also studied the process in the context of dynamic force spectroscopy (DFS). We performed DFS experiments by changing the temperature and cantilever spring constant, and analyzed the results in the context of the developed theories for DFS. We found that experiments performed at different temperatures and with different cantilever spring constants enabled a more effective comparison of experimental data with theory in comparison with previously published data.
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