ArticleFree radical biology & medicine2012
Ceruloplasmin (ferroxidase) oxidizes hydroxylamine probes: deceptive implications for free radical detection.
Article in Free radical biology & medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 27 citations in OpenAlex.
- Copper speciation in dairy cattle with different productivity levels.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2025Article
- Ceruloplasmin as a Vital Tool for Grading of Preeclampsia: A Case-Control Study in Central India.Journal of pharmacy & bioallied sciences · 2025Article
- The role of intracellular and extracellular copper compartmentalization in Alzheimer's disease pathology and its implications for diagnosis and therapy.Frontiers in neuroscience · 2025Review
- Zinc Supplementation Improves Texture, Oxidative Stability of Caciotta Cheese and Reduces Biogenic Amines Production.Animals : an open access journal from MDPI · 2024Article
- A Pilot Study on Oxidative Stress during the Recovery Phase in Critical COVID-19 Patients in a Rehabilitation Facility: Potential Utility of the PAOTBiomedicines · 2023Article
- Natural Antioxidant Activities of Plants in Preventing Cataractogenesis.Antioxidants (Basel, Switzerland) · 2022Review
- Molecular Functions of Ceruloplasmin in Metabolic Disease Pathology.Diabetes, metabolic syndrome and obesity : targets and therapy · 2022Review
- Trace elements and oxidative stress status in patients with psoriasis.Postepy dermatologii i alergologii · 2020Article
- Does Ceruloplasmin Defend Against Neurodegenerative Diseases?Current neuropharmacology · 2019Review
- Compartmentalized oxidative stress in dopaminergic cell death induced by pesticides and complex I inhibitors: distinct roles of superoxide anion and superoxide dismutases.Free radical biology & medicine · 2013Article
- Oxidative Stress in Peritoneal Dialysis Patients: Association with the Dialysis Adequacy and Technique Survival.Indian journal of nephrologyArticle
Corrections and comments
- Erratum issuedJang, JinJie [corrected to Jiang, JinJie]
Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
Ceruloplasmin (ferroxidase) is a copper-binding protein known to promote Fe(2+) oxidation in plasma of mammals. In addition to its classical ferroxidase activity, ceruloplasmin is known to catalyze the oxidation of various substrates, such as amines and catechols. Assays based on cyclic hydroxylamine oxidation are used to quantify and detect free radicals in biological samples ex vivo and in vitro. We show here that human ceruloplasmin promotes the oxidation of the cyclic hydroxylamine 1-hydroxy-3-carboxy-2,2,5,5-tetramethylpyrrolidine hydrochloride (CPH) and related probes in Chelex-treated phosphate buffer and rat serum. The reaction is suppressed by the metal chelators DTPA, EDTA, and desferal, whereas heparin and bathocuproine have no effect. Catalase or superoxide dismutase additions do not interfere with the CPH-oxidation yield, demonstrating that oxygen-derived free radicals are not involved in the CPH oxidation mediated by ceruloplasmin. Plasma samples immunodepleted of ceruloplasmin have lower levels of CPH oxidation, which confirms the role of ceruloplasmin (ferroxidase) as a biological oxidizing agent of cyclic hydroxylamines. In conclusion, we show that the ferroxidase activity of ceruloplasmin is a possible biological source of artifacts in the cyclic hydroxylamine-oxidation assay used for reactive oxygen species detection and quantification.
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