ArticleBiophysical journal2012
Dynamics and size of cross-linking-induced lipid nanodomains in model membranes.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 58 citations in OpenAlex.
- Nanodomain formation in lipid bilayers I: Quantifying the nanoscopic miscibility transition with FRET.Biochimica et biophysica acta. Biomembranes · 2026Article
- Nanodomain formation in lipid bilayers I: Quantifying the nanoscopic miscibility transition with FRET.bioRxiv : the preprint server for biology · 2026Article
- Which Moiety Drives Gangliosides to Form Nanodomains?The journal of physical chemistry letters · 2023Article
- Interleaflet organization of membrane nanodomains: What can(not) be resolved by FRET?Biophysical journal · 2023Article
- The ganglioside GM1a functions as a coreceptor/attachment factor for dengue virus during infection.The Journal of biological chemistry · 2022Article
- Role of lipid rafts in persistentAnnals of translational medicine · 2022Review
- Structured clustering of the glycosphingolipid GM1 is required for membrane curvature induced by cholera toxin.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Membrane Protein Dimerization in Cell-Derived Lipid Membranes Measured by FRET with MC Simulations.Biophysical journal · 2020Article
- Interleaflet Coupling of Lipid Nanodomains - Insights FromFrontiers in cell and developmental biology · 2020Review
- Article
- Nanoscale Membrane Budding Induced by CTxB and Detected via Polarized Localization Microscopy.Biophysical journal · 2017Article
- Impact of GMBiophysical journal · 2017Review
- Complex dynamics at the nanoscale in simple biomembranes.Scientific reports · 2017Article
- Lipid Driven Nanodomains in Giant Lipid Vesicles are Fluid and Disordered.Scientific reports · 2017Article
- Investigation of Nanoscopic Phase Separations in Lipid Membranes Using Inverse FCS.Biophysical journal · 2017Article
- Measuring the Invisible: Determining the Size of Growing Nanodomains Using the "Inverse FCS".Biophysical journal · 2017Article
- Glycolipid Crosslinking Is Required for Cholera Toxin to Partition Into and Stabilize Ordered Domains.Biophysical journal · 2016Article
- A protocol for the systematic and quantitative measurement of protein-lipid interactions using the liposome-microarray-based assay.Nature protocols · 2016Article
- Functions of cholera toxin B-subunit as a raft cross-linker.Essays in biochemistry · 2015Article
- Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Changes of membrane organization upon cross-linking of its components trigger cell signaling response to various exogenous factors. Cross-linking of raft gangliosides GM1 with cholera toxin (CTxB) was shown to cause microscopic phase separation in model membranes, and the CTxB-GM1 complexes forming a minimal lipid raft unit are the subject of ongoing cell membrane research. Yet, those subdiffraction sized rafts have never been described in terms of size and dynamics. By means of two-color z-scan fluorescence correlation spectroscopy, we show that the nanosized domains are formed in model membranes at lower sphingomyelin (Sph) content than needed for the large-scale phase separation and that the CTxB-GM1 complexes are confined in the domains poorly stabilized with Sph. Förster resonance energy transfer together with Monte Carlo modeling of the donor decay response reveal the domain radius of ~8 nm, which increases at higher Sph content. We observed two types of domains behaving differently, which suggests a dual role of the cross-linker: first, local transient condensation of the GM1 molecules compensating for a lack of Sph and second, coalescence of existing nanodomains ending in large-scale phase separation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.