ArticleBiophysical journal2012
Lipid sorting by ceramide and the consequences for membrane proteins.
Article in Biophysical journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 28 citations in OpenAlex.
- The Effect of Ceramide Ratio on the Membrane Curvature of Mimetic Models of Matrix Vesicles.ACS physical chemistry Au · 2025Article
- Ceramide signaling in immunity: a molecular perspective.Lipids in health and disease · 2025Review
- Influence of ceramide on lipid domain stability studied with small-angle neutron scattering: The role of acyl chain length and unsaturation.Chemistry and physics of lipids · 2022Article
- Extracellular Vesicles and Immune System in Ageing and Immune Diseases.Experimental neurobiology · 2021Review
- Sphingolipids in neurodegeneration (with focus on ceramide and S1P).Advances in biological regulation · 2018Review
- Sphingolipids and lipid rafts: Novel concepts and methods of analysis.Chemistry and physics of lipids · 2018Review
- Intrinsic Curvature-Mediated Transbilayer Coupling in Asymmetric Lipid Vesicles.Biophysical journal · 2018Article
- Role of Acid Sphingomyelinase in Shifting the Balance Between Proinflammatory and Reparative Bone Marrow Cells in Diabetic Retinopathy.Stem cells (Dayton, Ohio) · 2016Article
- Influence of Hydroxylation, Chain Length, and Chain Unsaturation on Bilayer Properties of Ceramides.Biophysical journal · 2015Article
- Bending Rigidities and Interdomain Forces in Membranes with Coexisting Lipid Domains.Biophysical journal · 2015Article
- How cholesterol could be drawn to the cytoplasmic leaf of the plasma membrane by phosphatidylethanolamine.Biophysical journal · 2014Article
- Monolayer spontaneous curvature of raft-forming membrane lipids.Soft matter · 2013Article
- Use of X-ray scattering to aid the design and delivery of membrane-active drugs.European biophysics journal : EBJ · 2012Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We mimicked the effect of sphingomyelinase activity on lipid mixtures of palmitoyl-oleoyl-phosphatidylcholine, sphingomyelin, ceramide, and 10 mol % cholesterol. Using x-ray diffraction experiments in combination with osmotic stress we found, in agreement with previous studies, that ceramide induces a coexistence of L(α) and L(β) domains. A detailed structural analysis of the coexisting domains demonstrated an increase of lipid packing density and membrane thickness in the L(α) domains upon increasing overall ceramide levels. This provides evidence for a ceramide-driven accumulation of cholesterol in the L(α) domains, in support of previous reports. We further determined the bending rigidities of the coexisting domains and found that the accumulation of cholesterol in the L(α) domains stabilizes their bending rigidity, which experiences a dramatic drop in the absence of cholesterol. Deriving experimental estimates for the spontaneous curvature and Gaussian modulus of curvature, we show, using a simple geometric model for ion channels, that in this way changes in the conformational equilibrium of membrane proteins can be kept small.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.