ArticleAnnals of clinical microbiology and antimicrobials2012
First description of Escherichia coli producing CTX-M-15- extended spectrum beta lactamase (ESBL) in out-patients from south eastern Nigeria.
Article in Annals of clinical microbiology and antimicrobials, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 57 citations in OpenAlex.
- Prevalence of CTX-M types among ESBL-producing pathogenicFood science and biotechnology · 2024Article
- Whole genome sequencing of Salmonella enterica serovars isolated from humans, animals, and the environment in Lagos, Nigeria.BMC microbiology · 2023Article
- EVALUATION OF PHENOTYPIC AND MOLECULAR TECHNIQUE IN THE DETECTION OF EXTENDED SPECTRUM BETA-LACTAMASE (ESBL)-PRODUCING GRAM NEGATIVE BACILLI IN OGUN STATE, NIGERIA.Annals of Ibadan postgraduate medicine · 2022Article
- Molecular characterization of multi drug resistant Escherichia coli isolates at a tertiary hospital in Abuja, Nigeria.Scientific reports · 2022Article
- Beta-lactamase resistance genes inAfrican journal of laboratory medicine · 2022Review
- Molecular characterization of extended spectrum cephalosporin resistantFrontiers in microbiology · 2022Article
- Prevalence of Extended-SpectrumInternational journal of microbiology · 2021Review
- Phenotypic and Molecular Characterization ofThe Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2020Article
- Molecular characterization of invasive Enterobacteriaceae from pediatric patients in Central and Northwestern Nigeria.PloS one · 2020Article
- Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in cattle production - a threat around the world.Heliyon · 2020Review
- A large self-transmissible resistance plasmid from Nigeria contains genes that ameliorate a carrying cost.Scientific reports · 2019Article
- Phenotypic and molecular characterization of antimicrobial resistantThe Pan African medical journal · 2019Article
- Phenotypic and Molecular Epidemiology of ESBL-, AmpC-, and Carbapenemase-ProducingFrontiers in microbiology · 2019Article
- Extended Spectrum Beta-Lactamase (ESBL)-producing bacteria isolated from hospital wastewaters, rivers and aquaculture sources in Nigeria.Environmental science and pollution research international · 2018Article
- Molecular detection of CTX-M-15-type β-lactamases in Escherichia coli strains from Senegal.New microbes and new infections · 2016Article
- Sequence analysis of genes mediating extended-spectrum beta-lactamase (ESBL) production in isolates of Enterobacteriaceae in a Lagos Teaching Hospital, Nigeria.BMC infectious diseases · 2015Article
- A prospective observational study of bacteraemia in adults admitted to an urban Mozambican hospital.South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde · 2015Observational
- Antibiotic resistance and genotype of beta-lactamase producing Escherichia coli in nosocomial infections in Cotonou, Benin.Annals of clinical microbiology and antimicrobials · 2015Article
- Phenotypic and Molecular Characterisation of Extended-Spectrum Beta-Lactamase Producing Escherichia coli Obtained from Animal Fecal Samples in Ado Ekiti, Nigeria.Journal of environmental and public health · 2015Article
- bla CTX-M-I group extended spectrum beta lactamase-producing Salmonella typhi from hospitalized patients in Lagos, Nigeria.Infection and drug resistance · 2015Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We studied the presence of extended spectrum beta lactamases (ESBLs) in 44 clinical isolates of Escherichia coli collected from out-patients in two university teaching hospitals in South-Eastern Nigeria. Species identification was performed by standard microbiology methods and re-confirmed by MALDI-TOF technology. Phenotypic characterization of ESBL enzymes was done by double disc synergy test and presence of ESBL genes was determined by specific PCR followed by sequencing. Transfer of plasmid DNA was carried out by transformation using E. coli DH5 as recipient strain. Phenotypic characterization identified all isolates to be ESBL positive. 77% of strains were from urine, 13.6% from vaginal swabs and 9.0% from wound swabs. 63.6% were from female patients, 68% were from outpatients and 95.5% from patients younger than 30 years. All ESBL producers were positive in a PCR for bla(CTX-M-1) cluster, in exemplary strains bla(CTX-M-15) was found by sequencing. In all strains ISEcp1 was found upstream and ORF477 downstream of bla(CTX-M). PCR for bla(TEM) and bla(OXA-1) was positive in 93.1% of strains, whereas bla(SHV) was not detected, aac(6')-Ib-cr was found in 97.7% of strains. RAPD analysis revealed seven different clonal groups named A through G with the majority of the strains (65.9%) belonging to clone A. Transfer of an ESBL plasmid with co-resistance to gentamicin, kanamycin, tobramycin, doxycycline and trimethropim-sulfamethoxazole was successful in 19 (43.2%) strains. This study showed a high rate of CTX-M-1 cluster - ESBLs in South-Eastern Nigeria and further confirms the worldwide spread of CTX-M ESBL in clinical isolates.
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