ArticleBMC research notes2012
Evaluation of qPCR reference genes in two genotypes of Populus for use in photoperiod and low-temperature studies.
Article in BMC research notes, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Article
- Overexpression of pxAlaAT3 in Populus × xiaohei alleviates root growth inhibition under ammonium nitrogen stress.Plant cell reports · 2025Article
- GAPDH Gene Family inInternational journal of molecular sciences · 2025Article
- Core clock genes adjust growth cessation time to day-night switches in poplar.Nature communications · 2024Article
- Transcriptomic and Physiological Analysis Reveals Genes Associated with Drought Stress Responses inPlants (Basel, Switzerland) · 2023Article
- Eucalyptus grandis AUX/INDOLE-3-ACETIC ACID 13 (EgrIAA13) is a novel transcriptional regulator of xylogenesis.Plant molecular biology · 2022Article
- Chemical and Molecular Characterization of Wound-Induced Suberization in Poplar (Plants (Basel, Switzerland) · 2022Article
- Abundance, efficiency, and stability of reference transcript expression in a seasonal rodent: The Siberian hamster.PloS one · 2022Article
- Vegetative phase change in Populus tremula × alba.The New phytologist · 2021Article
- Identification and validation of miRNA reference genes in poplar under pathogen stress.Molecular biology reports · 2021Article
- Identification and Characterization of theGenes · 2021Article
- FLOWERING LOCUS T2 Promotes Shoot Apex Development and Restricts Internode ElongationFrontiers in plant science · 2021Article
- Seasonal nitrogen remobilization and the role of auxin transport in poplar trees.Journal of experimental botany · 2020Article
- Genome-wide identification of3 Biotech · 2020Article
- Selection of Appropriate Reference Genes for Gene Expression Analysis under Abiotic Stresses inInternational journal of molecular sciences · 2019Article
- Selection and validation of reference genes for quantitative expression analysis of miRNAs and mRNAs in Poplar.Plant methods · 2019Article
- Recommendations for improving accuracy of gene expression data in bone and cartilage tissue engineering.Scientific reports · 2018Article
- Genome-Wide Constitutively Expressed Gene Analysis and New Reference Gene Selection Based on Transcriptome Data: A Case Study from Poplar/Canker Disease Interaction.Frontiers in plant science · 2017Article
- Article
- Enhancing digestibility and ethanol yield of Populus wood via expression of an engineered monolignol 4-O-methyltransferase.Nature communications · 2016Article
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3 authors.
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Abstract
backgroundQuantitative PCR (qPCR) is a widely used technique for gene expression analysis. A common normalization method for accurate qPCR data analysis involves stable reference genes to determine relative gene expression. Despite extensive research in the forest tree species Populus, there is not a resource for reference genes that meet the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) standards for qPCR techniques and analysis. Since Populus is a woody perennial species, studies of seasonal changes in gene expression are important towards advancing knowledge of this important developmental and physiological trait. The objective of this study was to evaluate reference gene expression stability in various tissues and growth conditions in two important Populus genotypes (P. trichocarpa "Nisqually 1" and P. tremula x P. alba 717 1-B4) following MIQE guidelines.
resultsWe evaluated gene expression stability in shoot tips, young leaves, mature leaves and bark tissues from P. trichocarpa and P. tremula. x P. alba grown under long-day (LD), short-day (SD) or SD plus low-temperatures conditions. Gene expression data were analyzed for stable reference genes among 18S rRNA, ACT2, CDC2, CYC063, TIP4-like, UBQ7, PT1 and ANT using two software packages, geNorm(PLUS) and BestKeeper. GeNorm(PLUS) ranked TIP4-like and PT1 among the most stable genes in most genotype/tissue combinations while BestKeeper ranked CDC2 and ACT2 among the most stable genes.
conclusionsThis is the first comprehensive evaluation of reference genes in two important Populus genotypes and the only study in Populus that meets MIQE standards. Both analysis programs identified stable reference genes in both genotypes and all tissues grown under different photoperiods. This set of reference genes was found to be suitable for either genotype considered here and may potentially be suitable for other Populus species and genotypes. These results provide a valuable resource for the Populus research community.
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