ArticleBMC genomics2012
Clostridium thermocellum ATCC27405 transcriptomic, metabolomic and proteomic profiles after ethanol stress.
Article in BMC genomics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 81 citations in OpenAlex.
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- Modulating the Immunosuppressive Tumor Microenvironment and Inhibiting Growth in Mutp53-Driven CRPC via STAT3 Pathway Blockade.International journal of biological sciences · 2025Article
- Ethanol tolerance in engineered strains of Clostridium thermocellum.Biotechnology for biofuels and bioproducts · 2023Article
- Ethanol tolerance of Clostridium thermocellum: the role of chaotropicity, temperature and pathway thermodynamics on growth and fermentative capacity.Microbial cell factories · 2022Article
- Utilization of Monosaccharides byMicroorganisms · 2021Article
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- Genome-Wide Transcription Factor DNA Binding Sites and Gene Regulatory Networks inFrontiers in microbiology · 2021Article
- Transcriptomic analysis of a Clostridium thermocellum strain engineered to utilize xylose: responses to xylose versus cellobiose feeding.Scientific reports · 2020Article
- Quantitative proteomic analysis reveals the ethanologenic metabolism regulation ofBiotechnology for biofuels · 2019Article
- Characterization of a Dimeric Arginase FromFrontiers in microbiology · 2019Article
- Large Noncoding RNAs in Bacteria.Microbiology spectrum · 2018Review
- A machine learning approach to predict metabolic pathway dynamics from time-series multiomics data.NPJ systems biology and applications · 2018Article
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- Specialized activities and expression differences for Clostridium thermocellum biofilm and planktonic cells.Scientific reports · 2017Article
- Integrated omics analyses reveal the details of metabolic adaptation ofBiotechnology for biofuels · 2017Article
- Metabolome analysis reveals a role for glyceraldehyde 3-phosphate dehydrogenase in the inhibition ofBiotechnology for biofuels · 2017Article
- Development of a coreBiotechnology for biofuels · 2017Article
- Precise pretreatment of lignocellulose: relating substrate modification with subsequent hydrolysis and fermentation to products and by-products.Biotechnology for biofuels · 2017Article
- Improved growth rate inBiotechnology for biofuels · 2017Article
- Transcriptomic and proteomic analyses of core metabolism in Clostridium termitidis CT1112 during growth on α-cellulose, xylan, cellobiose and xylose.BMC microbiology · 2016Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundClostridium thermocellum is a candidate consolidated bioprocessing biocatalyst, which is a microorganism that expresses enzymes for both cellulose hydrolysis and its fermentation to produce fuels such as lignocellulosic ethanol. However, C. thermocellum is relatively sensitive to ethanol compared to ethanologenic microorganisms such as yeast and Zymomonas mobilis that are used in industrial fermentations but do not possess native enzymes for industrial cellulose hydrolysis.
resultsIn this study, C. thermocellum was grown to mid-exponential phase and then treated with ethanol to a final concentration of 3.9 g/L to investigate its physiological and regulatory responses to ethanol stress. Samples were taken pre-shock and 2, 12, 30, 60, 120, and 240 min post-shock, and from untreated control fermentations for systems biology analyses. Cell growth was arrested by ethanol supplementation with intracellular accumulation of carbon sources such as cellobiose, and sugar phosphates, including fructose-6-phosphate and glucose-6-phosphate. The largest response of C. thermocellum to ethanol shock treatment was in genes and proteins related to nitrogen uptake and metabolism, which is likely important for redirecting the cells physiology to overcome inhibition and allow growth to resume.
conclusionThis study suggests possible avenues for metabolic engineering and provides comprehensive, integrated systems biology datasets that will be useful for future metabolic modeling and strain development endeavors.
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